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目的:观察红景天苷(SDS)对脂多糖(LPS)引起的大鼠急性肺损伤(ALI)的治疗作用,并初步探讨其作用的机制。方法:将36只SD大鼠随机分为生理盐水(NS)对照组、LPS组和LPS+SDS组(每组n=12),通过向气管内滴注LPS建立大鼠ALP模型。采用微量注射泵向大鼠右颈外静脉注射液体并留置的给药方法,NS组和LPS组注射NS并留置4 h;LPS+SDS组先于气管内滴注LPS,0.5 h后再注射SDS并留置4 h。4.5 h后处死实验动物,取肺组织观察其形态学改变,测定肺湿/干质量的比值(W/D)、肺泡灌洗液(BALF)中蛋白的含量及肺组织匀浆中TNF-α、IL-6、IL-10、乳酸脱氢酶(LDH)和髓过氧化物酶(MPO)的含量。结果:形态学观察表明,LPS组肺组织水肿,有点、片状出血及大量的炎性细胞浸润,肺泡间隔显著增厚,肺泡腔变窄、结构消失。LPS+SDS组的肺组织损伤明显减轻,肺组织结构趋于正常,肺泡腔及支气管腔炎细胞及渗出物与LPS组相比明显减少。LPS组肺W/D与NS组相比明显增加(P<0.05),BALF中蛋白的含量显著增加(P<0.05),肺组织匀浆中TNF-α、IL-6、IL-10、LDH和MPO的含量显著增加(P<0.05);而给予SDS治疗后,肺W/D、BALF中蛋白含量、肺组织匀浆中TNF-α、IL-6、LDH和MPO的含量与LPS组相比均明显减少(P<0.05),IL-10含量显著增加(P<0.05)。结论:SDS对LPS所致ALI具有治疗作用,这种治疗作用可能与其通过抑制肺组织中炎症介质的作用有关。
Objective: To observe the therapeutic effect of salidroside (SDS) on lipopolysaccharide (LPS) -induced acute lung injury (ALI) in rats and to explore its mechanism. Methods: Thirty-six Sprague-Dawley rats were randomly divided into normal saline (NS) control group, LPS group and LPS + SDS group (n = 12 each). ALP model was established by intratracheal instillation of LPS. The rats in NS group and LPS group were injected with NS and left for 4 hours. The LPS + SDS group was given LPS intratracheal instillation first, followed by 0.5 hour injection of SDS And stay 4 h. After 4.5 h, the experimental animals were sacrificed and the lungs were taken out to observe the morphological changes. The lung wet / dry weight ratio (W / D), the content of alveolar lavage fluid (BALF) and the content of TNF-α , IL-6, IL-10, lactate dehydrogenase (LDH) and myeloperoxidase (MPO). Results: Morphological observation showed that lung tissue edema, a little, flaky hemorrhage and a large number of infiltration of inflammatory cells in LPS group significantly increased alveolar septa, narrowed alveolar space and disappeared. LPS + SDS group lung tissue injury was significantly reduced, the lung tissue tends to be normal, alveolar and bronchial cavity inflammatory cells and exudate compared with the LPS group was significantly reduced. The lung W / D in LPS group was significantly higher than that in NS group (P <0.05), and the content of protein in BALF was significantly increased (P <0.05). The levels of TNF-α, IL-6, IL-10, (P <0.05). After the treatment with SDS, the content of protein in lung W / D and BALF, the levels of TNF-α, IL-6, LDH and MPO in lung homogenate were significantly lower than those in LPS group (P <0.05), IL-10 content increased significantly (P <0.05). Conclusion: SDS has a therapeutic effect on ALI induced by LPS, which may be related to the inhibition of inflammatory mediators in lung tissue.