【目的】了解Cd胁迫及增施Fe对不同柳树生长及光合生理的影响,探讨增施Fe对降低柳树Cd毒害和提高Cd耐性以及对柳树Cd修复的Fe调控作用。【方法】以旱柳‘Levante’和蒿柳无性系79026及790260为材料,利用1/2 Hoagland营养液(含25μmol/L EDTA-Na_2Fe)培养法,以不同Cd浓度(0、10、50、100μmol/L)(不增施Fe)和上述Cd浓度下增施Fe(25μmol/L)两组处理,培养42 d后测定不同柳树生长量(苗高和地径)、干物质量(根、茎、叶)、光合作用指标(叶绿素a、b含量、叶绿素a/b、净光合速率、气孔导度和胞间CO_2浓度)和Cd、Fe含量(根、茎、叶)。【结果】(1)随着Cd浓度增大,3个无性系/品种生长量、干物质量、叶绿素a、b含量、净光合速率、气孔导度和Fe含量(茎、叶)减少,叶绿素a/b、胞间CO_2浓度、Cd含量(根、茎、叶)增大,但根Fe含量在低中浓度Cd(10、50μmol/L)时逐渐增大,在高浓度Cd(100μmol/L)时减少。在相同Cd浓度胁迫下,3个无性系/品种Cd和Fe含量大小为根﹥叶﹥茎,旱柳根Cd含量大于蒿柳,但叶Cd含量小于蒿柳。(2)与低浓度Cd时增施Fe相比,在中高浓度Cd时增施Fe后,3个无性系/品种叶绿素a、b含量、旱柳‘Levante’和蒿柳79026生长量和干物质量(旱柳‘Levante’茎除外)、旱柳‘Levante’叶绿素a/b和叶片净光合速率均显著减小,而3个无性系/品种Cd含量增大,Fe含量减少。在高浓度Cd时增施Fe后旱柳‘Levante’和蒿柳790260叶Cd含量均小于中浓度Cd时增施Fe的,且旱柳Cd含量(根、茎、叶)和Fe含量(叶)均小于蒿柳。(3)增施Fe提高了Cd胁迫下柳树生长和干物质量、叶绿素a、b含量、气孔导度及胞间CO_2浓度,特别是在高浓度Cd时增施Fe后降低了3个无性系/品种根和叶Cd含量,增加了茎Cd含量,旱柳Cd含量(根、茎和叶)均小于蒿柳。【结论】Cd胁迫抑制了柳树生长和干物质积累以及叶绿素合成、光合作用,在中、高浓度Cd胁迫时,对旱柳生长和干物质量积累的抑制作用大于蒿柳;柳树根的Cd和Fe积累能力大于茎和叶,蒿柳Cd积累能力大于旱柳。增施Fe提高了Cd胁迫下柳树生长量和干物质量以及叶绿素含量,不同程度地增加了Cd含量和Fe含量,一定程度上缓解了Cd胁迫对柳树光合作用的影响,且对旱柳的影响作用大于蒿柳,这证实了Cd胁迫下增施Fe改善Cd胁迫下柳树对生长和生理的适应性。 【Objective】 The objective of this study was to understand the effects of Cd stress and Fe addition on the growth and photosynthesis of different willows, and to explore the effects of increasing Fe on the Cd toxicity and Cd tolerance and the regulation of Fe on Willow Cd. 【Method】 With the methods of ½ Hoagland nutrient solution (containing 25μmol / L EDTA-Na_2Fe) and different Cd concentrations (0, 10, 50, 100μmol / L) and Fe (25μmol / L) at the above Cd concentration. After 42 days of culture, the growth of different willow species (seedling height and diameter), dry matter weight , Leaf), photosynthesis indicators (chlorophyll a, b content, chlorophyll a / b, net photosynthetic rate, stomatal conductance and intercellular CO 2 concentration) and Cd, Fe content (roots, stems and leaves). 【Result】 (1) With the increase of Cd concentration, the growth, dry matter content, chlorophyll a, b content, net photosynthetic rate, stomatal conductance and Fe content (stems and leaves) of three clones / / b, the content of intercellular CO 2 and Cd increased (root, stem and leaf), but the root Fe content increased gradually with low and medium Cd (10,50μmol / L) Decrease Under the same Cd stress, the content of Cd and Fe in three clones / cultivars was root> leaf> stem, and the content of Cd in the roots of A. solanacearum was higher than that of Artemisia argentea. (2) The contents of chlorophyll a, b and chlorophyll a, b, the growth and the dry matter content of three kinds of Auxiliuon and Artemisia lauren 79026 were increased after the application of Fe at medium and high concentrations of Cd, (Except for “Levante” stems), chlorophyll a / b and leaf net photosynthetic rate of ’Levante’ were significantly reduced, while the Cd content of three clones / cultivars increased and Fe content decreased. The results showed that Cd content (root, stem, leaf) and Fe content (leaf) of H. amurense and A. lauren 790260 were higher than those of middle Cd when Fe was applied at high Cd concentration. Are less than Artemisia. (3) Fe addition increased the growth and dry matter content, chlorophyll a, b content, stomatal conductance and intercellular CO 2 concentration of willow under cadmium stress, especially decreased the three clones / The content of Cd in roots and leaves of cultivars increased Cd content in the stems, and the content of Cd in the roots (roots, stems and leaves) was less than that of Artemisia. 【Conclusion】 Cd stress inhibited the growth and dry matter accumulation, chlorophyll synthesis and photosynthesis of willow and inhibited the growth and dry matter accumulation of A. amurense under medium and high Cd stress. Cd and Fe Accumulation capacity is greater than the stems and leaves, Artemisia candel Cd accumulation ability than the willow. The addition of Fe increased the growth, dry matter and chlorophyll content of willow under Cd stress, increased Cd and Fe contents to some extent, and alleviated the effects of Cd stress on the photosynthesis of willow to some extent, Which was higher than Artemisia annua, which confirmed that the application of Fe under Cd stress improved the adaptability of willow to growth and physiology under Cd stress.