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在河北省搜集了东北部山区、东北部平原、白洋淀湖泊区、西南丘陵山区等 18个天然野生大豆群体的 4 6 1份植株种子样本 ,调查其Kunitz型胰蛋白酶抑制剂蛋白 (KTI)的形态及蛋白位点在野生大豆天然个体群体及地理的频率变化。结果显示超过 5 0 %个体群体的KTI位点是多态的 ,18个天然个体群体中 12个群体形态是由Tia和Tib构成的混合群体。这次测定的河北野生大豆中没有发现Tic形态存在。在 12个混合群体中 ,4个群体发现存在Tia/Tib杂合种子 ,约占调查群体的 2 2 % ,在野生大豆总群体中至少有 1%的异交率。河北天然野生大豆Tia基因频率约占 85 % ,Tib约 15 %。地理上 ,东北部山区和平原生态区比南部的白洋淀和西南丘陵山地生态区有较高的Tib频率 ,无论在南部或北部 ,山区生态区Tib频率高于平原生态区 ,同时山区生态区内的个体群体间的基因频率差异变化程度高于平原群体间的变化 ;个体群体的基因频率没有看到与地理距离有相关性。白洋淀湖泊生态区虽然有河北最大的野生群落 ,但是KTI位点是Tia单态的 ,湖内和湖外群体KTI位点是一致的单态性 ,暗示白洋淀区域野生群体间受湖水流动的影响 ,使Tia型种子在群体传播形成一个单态优势型 ,也许这个区域的野生大豆遗传背景较其它地区群体狭窄。在这个研究中 ,我们还首?
A total of 461 plant seed samples from 18 natural wild soybean populations including northeastern mountainous area, northeastern plain, Baiyangdian Lake Area and southwest hills and hills were collected in Hebei Province. The morphology of Kunitz-type trypsin inhibitor protein (KTI) And protein sites in wild soybean natural individuals and geographical frequency changes. The results showed that the KTI loci of more than 50% of the individuals were polymorphic, and 12 of the 18 native individuals were a mixed population of Tia and Tib. This determination of Hebei wild soybean did not find the presence of Tic form. Among the 12 mixed populations, 4 populations found Tia / Tib hybrid seeds, accounting for about 22% of the surveyed population and at least 1% of the total wild soybean populations. The Tia gene frequency of Hebei native wild soybean accounts for about 85% and Tib about 15%. Geographically, the northeastern mountainous plain and the plain ecological zone have a higher Tib frequency than the Baiyangdian and the southwest hilly mountainous ecological zones in the south. Both in the southern and northern part, the Tib frequency in the mountainous ecological zone is higher than that in the plain ecological zone, meanwhile, The frequency of genetic variation among individual groups was higher than that among plain groups. The genetic frequency of individual groups did not show any correlation with geographic distance. Although there is the largest wild community in Hebei Baiyangdian lakes ecological zone, the KTI locus is Tia monomorphic, and the KTI loci of lake and lakes are consistent with monosomicity, which implies that the wild populations in Baiyangdian Lake area are affected by the flow of lakes. The Tia-type seeds spread in the population to form a single-dominance type, and the genetic background of the wild soybean in this area may be narrower than that in other regions. In this study, we also first?