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肿瘤细胞和胞外基质之间的黏附特性与肿瘤的侵袭转移有密切关系。作者从细胞周期的角度,采用微管吸吮技术和细胞同步技术研究了不同细胞周期肝癌细胞与I型胶原裱衬表面的黏附力学特性。结果表明:胸腺嘧啶脱氧核苷、秋水仙碱顺序阻断和胸腺嘧啶脱氧核苷双阻断后释放培养的方法可分别获得G 1期和S期肝癌细胞,平均同步率分别为74.09%和90.39%;在研究剂量和时间范围内,肝癌细胞与I型胶原的黏附力具有浓度和时间依赖性;S期肝癌细胞和I型胶原的黏附力值与G 1期和未同步组(对照组)相应值比较明显降低。结果提示:肝癌细胞经血道转移的侵蚀细胞间质阶段,G 1期细胞可能起更重要的作用。这一研究对全面认识肝癌的转移机理有重要意义。
The adhesion between tumor cells and extracellular matrix is closely related to tumor invasion and metastasis. From the perspective of cell cycle, the authors used microtubule sucking technique and cell synchronization technique to study the adhesion mechanics characteristics of different cell cycle hepatocellular carcinoma cells and type I collagen-laden surface. The results showed that G 1 and S phase hepatoma cells could be obtained respectively by thymidine, cisbactate blockade and thymidine releasing after double-block, the average synchronization rates were 74.09% and 90.39 %. Adhesion of hepatocellular carcinoma cells to type I collagen was dose-dependent and time-dependent at the dose and time of study. The adhesion values of S phase hepatoma cells and type I collagen were similar to those of G 1 and unsynchronized groups (control group) The corresponding value is significantly reduced. The results suggest that G 1 phase cells may play a more important role in the interstitial stage of hepatocellular carcinoma cell migration by hematogenous metastasis. This research is of great significance to fully understand the mechanism of liver cancer metastasis.