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目的观察心理疗法对宫颈癌化疗患者血清中C-myc和PTEN mRNA水平的影响。方法选择同期体检健康中年妇女20例作为对照组;58例来医院化疗的宫颈癌患者作为实验组,再随机分为常规护理组20例和心理护理组38例,两组均于化疗第1天及化疗第21天后清晨空腹采静脉血。采用实时荧光定量法检测C-myc和PTEN mRNA的表达水平。结果化疗前与对照组比较,常规护理组和心理护理组患者血清C-myc mRNA的表达水平均明显升高、PTEN mRNA的表达水平均明显降低,差异有统计学意义(P<0.01);常规护理组和心理护理组组间差异无统计学意义(P>0.05)。化疗后与化疗前比较,常规护理组和心理护理组患者血清C-myc mRNA的表达水平均明显降低、PTEN mRNA的表达水平均明显升高,差异有统计学意义(P<0.01);心理护理组下调C-myc mRNA水平及上调PTEN mRNA水平好于常规护理组,差异有统计学意义(P<0.05)。结论心理护理比常规护理能更好的下调C-myc mRNA水平、上调PTEN mRNA水平,这可能是能提高宫颈癌化疗后生存率的有效手段之一。
Objective To observe the effects of psychological therapy on serum C-myc and PTEN mRNA levels in patients with cervical cancer. Methods Twenty healthy middle-aged women in the same period were selected as the control group. Fifty-eight patients with cervical cancer who were hospitalized for chemotherapy were randomly divided into two groups: conventional nursing group (20 cases) and psychological nursing group (38 cases) Day and chemotherapy after 21 days fasting venous blood. Real-time fluorescence quantitative detection of C-myc and PTEN mRNA expression levels. Results Compared with the control group, the levels of serum C-myc mRNA and the expression of PTEN mRNA in the normal nursing group and the psychological nursing group were significantly lower than those in the control group (P <0.01) There was no significant difference between nursing group and psychological nursing group (P> 0.05). The levels of serum C-myc mRNA and the expression of PTEN mRNA in normal nursing group and psychological nursing group were significantly lower than those before chemotherapy (P <0.01). Psychological nursing The level of C-myc mRNA and the level of PTEN mRNA in HCC group were significantly lower than those in routine nursing group (P <0.05). Conclusions Psychological nursing can better down-regulate C-myc mRNA expression and up-regulate the expression of PTEN mRNA than conventional nursing care, which may be one of the effective ways to improve the survival rate of cervical cancer after chemotherapy.