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目的:考察枸杞多糖(LBP)对多柔比星(DOX)作用下H9c2心肌细胞损伤的保护作用。方法:应用H9c2心肌细胞筛查体系,通过检测细胞存活率、过氧化物岐化酶(SOD)活性和氧化代谢产物(MDA)含量,考察LBP从33μg/mL至0.46 ng/mL浓度范围内细胞保护作用的最佳浓度。研究在最优浓度下,LBP对暴露于10、2和0.4μg/mL浓度DOX下H9c2心肌细胞的存活率的影响。应用A549肺癌细胞,考察LBP对DOX抗肿瘤活性的影响。结果:LPB的浓度达到1.85μg/mL时,实验体系下H9c2细胞存活率达到最大,同时可以改善DOX所引起的心肌细胞SOD活性降低和MDA浓度增高。在该浓度下,LBP可以有效地增加2和0.4μg/mL浓度DOX作用下心肌细胞的存活率;但不降低DOX对A549肿瘤细胞的杀伤效果。结论:LBP通过减轻氧化应激损伤降低DOX对心肌细胞的毒性作用。
Objective: To investigate the protective effect of LBP on H9c2 cardiomyocyte injury induced by doxorubicin (DOX). Methods: H9c2 cardiomyocyte screening system was used to detect the cell viability, the activity of superoxide dismutase (SOD) and the content of oxidative metabolite (MDA) in the cells of LBP from 33μg / mL to 0.46 ng / mL The best concentration of protection. The effects of LBP at optimal concentrations on the viability of H9c2 cardiomyocytes exposed to DOX concentrations of 10, 2 and 0.4 μg / mL were studied. A549 lung cancer cells were used to investigate the effect of LBP on the antitumor activity of DOX. Results: When the concentration of LPB reached 1.85μg / mL, the survival rate of H9c2 cells reached the maximum under the experimental system. At the same time, the decrease of SOD activity and the increase of MDA concentration in DOX-induced cardiomyocytes were also observed. At this concentration, LBP can effectively increase the survival rate of cardiomyocytes under DOX concentration of 2 and 0.4μg / mL; however, it did not reduce the killing effect of DOX on A549 tumor cells. Conclusion: LBP can reduce the toxicity of DOX to cardiomyocytes by reducing oxidative stress.