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目的研究苄基四氢巴马汀 (BTHP)和甲氧胺对豚鼠心室肌细胞上延迟整流钾电流作用。方法采用全细胞膜片钳技术测定延迟整流钾电流 (Ik)。结果甲氧胺 5 0 μmol·L- 1 在 37℃时可以分别使IK和IK ,tial从 (12 4± 1 8)pApF- 1 和 (4 2±0 5 )pApF- 1 上升至 (2 0 5± 0 7)pApF- 1 和 (7 2± 0 6 )pApF- 1 。若事先甲氧胺 5 0 μmol·L- 1 后 ,再加入BTHP 30 μmol·L- 1 可使BTHP阻滞IK和IK ,tial百分率分别从单独使用BTHP时的 37 5 %± 6 0 %和 35 9± 5 5 %上升到 6 2 9%± 4 3%和 6 1 1%±3 7%。结论苄基四氢巴马汀可以逆转甲氧胺对豚鼠心室肌细胞上IK 的增加作用。
Objective To study the effect of benzyl tetrahydropalmatine (BTHP) and methoxyamine on delayed rectifier potassium current in guinea pig ventricular myocytes. Methods Whole cell patch clamp technique was used to measure delayed rectifier potassium current (Ik). Results Methoxyamine 50 μmol·L -1 increased IK and IK, tial from (12 4 ± 1 8) pApF-1 and (42 ± 0 5) pApF-1 to (20 5 ± 0 7) pApF-1 and (7 2 ± 0 6) pApF-1. The BKP blockade IK, IK and tial percentage were increased from 37.5% ± 60% and 35% respectively when BTHP was treated with 30 μmol·L-1 B 9 ± 5 5% to 6 2 9% ± 4 3% and 6 1 1% ± 3 7%. Conclusion Benzyltetrahydropalmatine can reverse the increasing effect of methoxyamine on IK in guinea pig ventricular myocytes.