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目的 :探讨钾离子通道开放剂 (Nicorandil)对老年大鼠心肌缺血再灌注损伤的保护作用。方法 :在老年大鼠离体心脏的改良工作模型上 ,观察St.Thomas液 (S组 )、含 2 0 μmol/L钾离子通道开放剂Nicorandil的超极化停搏液 (N组 )和Nicorandil强化的St.Thomas液 (S +N组 )对老年大鼠进行低温缺血再灌注后的保护效果。结果 :缺血再灌注 30min后冠脉流量 (CF)恢复百分率、左室压力微分 (±dp/dt)及收缩压、舒张压恢复百分率S +N组明显优于S、N组 (P <0 0 5 ) ;灌注停跳液后心肌电活动和机械活动的消失时间S组少于N、S +N组 (P <0 0 1) ;再灌注后乳酸脱氢酶 (LDH)、磷酸肌酶同工酶(CK MB)漏出量及心肌含水量 3组间无明显差异。结论 :Nicorandil可增强老年大鼠心肌在缺血再灌注后的左室功能恢复 ,但延长心肌电、机械活动消失时间。因此 ,它对老年心肌的保护作用尚有待进一步研究。
Objective: To investigate the protective effects of Nicorandil on myocardial ischemia-reperfusion injury in aged rats. Methods: The hyperpolarized cardioplegia (N group) with Nicorandil (20 μmol / L potassium channel opener) and Nicorandil Fortified St. Thomas liquid (S + N group) protects the aged rats from hypothermia and reperfusion injury. Results: The percentage of recovery of coronary flow (CF), differential pressure of left ventricular pressure (± dp / dt) and systolic blood pressure and the percentage of recovery of diastolic blood pressure in group S + N after 30 min of ischemia / reperfusion were significantly better than those of group S and N (P <0.01). After reperfusion, the activity of LPS and the disappearance of mechanical activity in S group were less than those in N and S + N group (P <0.01). After reperfusion, lactate dehydrogenase (LDH) There was no significant difference between enzyme MBK leakage and myocardial water content in the three groups. Conclusion: Nicorandil can enhance the recovery of left ventricular function in the aged rat myocardium after ischemia-reperfusion, but prolong the disappearance of electrocardiogram and mechanical activity. Therefore, its protective effect on senile myocardium remains to be further studied.