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目的:探讨邻苯二甲酸二丁酯(DBP)对大鼠精子的生成和质量影响。方法:150只雄性大鼠,随机分为DBP低剂量组(50 mg·kg-1)、DBP中剂量组(200 mg·kg-1)、DBP高剂量组(1 000 mg·kg-1)、空白对照组(蒸馏水)和溶剂对照组(花生油),每组30只,每天灌胃给药,每30d每组取10只大鼠解剖取样,称量睾丸、附睾质量,取一侧附睾进行精子计数、精子存活率和精子形态分析。结果:灌胃90 d,DBP中、高剂量组精子数、精子存活率、睾丸、附质重量及其脏器系数均明显降低(P<0.05或0.01)。结论:长期大剂量使用DBP对大鼠生殖功能有毒性作用。
Objective: To investigate the effect of dibutyl phthalate (DBP) on sperm production and quality in rats. Methods: 150 male rats were randomly divided into low dose DBP group (50 mg · kg -1), DBP medium dose group (200 mg · kg -1) and DBP high dose group (1 000 mg · kg -1) , Control group (distilled water) and solvent control group (peanut oil), 30 rats in each group were given intragastric administration. Every 30 days, 10 rats in each group were dissected and sampled for testis and epididymis quality. Sperm count, sperm viability and sperm morphology analysis. Results: The sperm count, sperm survival rate, testis weight and the weight of organ and their organ coefficient decreased significantly (P <0.05 or 0.01). Conclusion: Long-term high-dose DBP has a toxic effect on reproductive function in rats.