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目的：研究不同表皮分离方法对大鼠表皮水渗透屏障功能的影响。方法：用胰蛋白酶法、Ｄｉｓｐａｓｅ法、加热法和一天培养－加热法四种不同方法从新生大鼠皮肤上分离得到大鼠表皮，在气－液面培养的不同阶段测定表皮的水渗透屏障系数并与新生大鼠皮肤的屏障功能相比较。结果：表皮水渗透系数（Ｋｐ）值在胰蛋白酶法为（２．１±０．９）ｃｍ·ｈ－１，Ｄｉｓｐａｓｅ法为（３．８±１．２）ｃｍ·ｈ－１，加热法为（４．３±１．４）ｃｍ·ｈ－１，一天培养－加热法为（２．２±０．７）ｃｍ·ｈ－１，而新生大鼠皮肤的Ｋｐ值为（１．９±０．９）ｃｍ·ｈ－１。Ｄｉｓｐａｓｅ法和加热法有可能损伤表皮的屏障结构导致表皮水渗透性增高。此种损伤经过约３ｄ的气－液面培养后即可得到部分的修复。在气－液面培养条件下，表皮正常的屏障功能可维持８～１０ｄ。结论：胰蛋白酶和一天培养－加热分离法对大鼠表皮屏障功能无明显损伤作用。在气－液面培养条件下，该表皮可维持正常的屏障功能
Objective: To study the effect of different epidermal isolation on the water barrier function of epidermis in rats. Methods: The epidermis of rats was isolated from the skin of neonatal rats by four different methods: trypsin method, Dispase method, heating method and one-day culture-heating method. The water barrier coefficient of epidermis was determined at different stages of gas-liquid culture And compared with the barrier function of neonatal rat skin. Results: The water permeation coefficient (Kp) of epidermis was (2.1 ± 0.9) cm · h-1 by trypsin method and (3.8 ± 1.2) cm · h-1 by Dispase method. (4.3 ± 1.4) cm · h-1, and the daily culture-heating method was (2.2 ± 0.7) cm · h-1, while the Kp value of neonatal rat skin was (1.9 ± 0.9) cm · h-1. Dispase method and heating method may damage the epidermal barrier structure leading to increased epidermal water permeability. After about 3 days, this kind of damage can be partially repaired by gas-liquid culture. In the gas - liquid culture conditions, the normal barrier function of the epidermis can be maintained 8 ~ 10d. Conclusion: Trypsin and one-day culture-heating method have no effect on the epidermal barrier function in rats. In gas-liquid culture conditions, the epidermis can maintain normal barrier function