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目的分析慢性乙型病毒性肝炎患者外周血树突状细胞亚群(DC1和DC2)相对数量特点。方法采集健康人和慢性乙型肝炎患者外周静脉抗凝全血,利用荧光抗体标记和流式细胞仪检测外周血树突状细胞亚群,DC1的特异性标记为Lineage-HLA-DR+CD11c+,DC2的特异性标记为Lineage-HLA-DR+CD123+;电化学发光检测血清乙型肝炎病毒标志。结果慢性乙型肝炎患者外周血DC2相对数量高于健康人近两倍,但缺少统计学意义,DC1相对数量在两者间没有差别;血清HBeAg阴性慢性乙型肝炎患者外周血DC2相对数量显著增高(P=0.002),差异存在于与HBeAg阳性患者和健康人比较中(分别D1,2=129,P<0.01;D2,3=108,P<0.05),而在上述3组中DC1相对数量无差异(P=0.183)。结论慢性乙型肝炎患者外周血DC2增加与HBeAg血清转换有关,提示DC2可能有抑制病毒的作用。
Objective To analyze the relative quantity of peripheral blood dendritic cell subsets (DC1 and DC2) in patients with chronic hepatitis B virus. Methods Peripheral anticoagulated whole blood was collected from healthy volunteers and patients with chronic hepatitis B. The dendritic cell subsets of peripheral blood were detected by fluorescent antibody and flow cytometry. The specific marker of DC1 was Lineage-HLA-DR + CD11c + The specificity of DC2 was labeled as Lineage-HLA-DR + CD123 +; Electrochemiluminescence was used to detect the serum hepatitis B virus marker. Results The relative amount of DC2 in peripheral blood of patients with chronic hepatitis B was nearly double that of healthy people, but there was no statistical significance. The relative quantity of DC1 did not differ between the two groups. The relative quantity of DC2 in peripheral blood of patients with HBeAg-negative chronic hepatitis B was significantly higher (P = 0.002). Differences were present in HBeAg-positive and healthy individuals (D1, 2 = 129, P <0.01; No difference (P = 0.183). Conclusion The increase of DC2 in peripheral blood of patients with chronic hepatitis B is related to the conversion of HBeAg seroconversion, suggesting that DC2 may inhibit the virus.