Ⅱ型肺泡上皮细胞与Ⅰ型肺泡上皮细胞在肺泡壁上掺杂存在,前者为肺泡上皮总数的14.5%,其覆盖面积只占肺泡总面积的3%。我们用Wistar大鼠,冲洗净肺内血液,尽量清除肺泡腔内巨噬细胞,向肺泡腔内灌注0.5%胰蛋白酶,制成匀浆,过滤离心,残渣涂片,用丹宁酸和巴氏染色变法标记,并用透射电镜、扫描电镜观察。 Type II alveolar epithelial cells and type I alveolar epithelial cells in the alveolar wall doping exist, the former is 14.5% of the total number of alveolar epithelia, covering only 3% of the total area of ​​the alveoli. We use Wistar rats, flushing the blood in the lungs, removing alveolar macrophages as much as possible, perfusing 0.5% trypsin into the alveolar space, homogenizing, centrifuging, smearing the residue, Staining method of labeling, and transmission electron microscopy, scanning electron microscopy.