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[目的]探讨马鞭草C部位对人绒癌JAR细胞抑制作用机理。[方法]通过相差显微镜观察活细胞贴壁程度、细胞形态改变;荧光显微镜观察细胞形态学变化;透射电镜观察细胞超微结构变化;琼脂糖凝胶电泳观察用药后细胞DNA断裂情况;流式细胞仪检测细胞凋亡情况。[结果]马鞭草C部位可引起细胞数目减少并萎缩;荧光显微镜下观察到典型凋亡细胞的形态学特征;电镜下见到明显的凋亡小体;琼脂糖凝胶电泳出现“阶梯状条带”;流式细胞仪检测发现用药后出现明显的凋亡峰。[结论]马鞭草C部位对人绒癌JAR细胞抑制作用机理是诱导其凋亡。
[Objective] To investigate the inhibitory mechanism of verbena C site on human choriocarcinoma JAR cells. [Method] The degree of attachment of living cells was observed by phase-contrast microscopy. Morphological changes of cells were observed by fluorescence microscopy. The ultrastructural changes of cells were observed by transmission electron microscopy. DNA fragmentation was observed by agarose gel electrophoresis. Flow cytometry Instrument detection of apoptosis. [Result] The number of cells in Verbena decreased and contracted. The morphological characteristics of typical apoptotic cells were observed under fluorescence microscope. The apoptotic bodies were observed under electron microscope. The “ladder” shape appeared on agarose gel electrophoresis Banding "; flow cytometry showed significant apoptotic peak after treatment. [Conclusion] The inhibitory mechanism of verbena C site on human choriocarcinoma JAR cells is induced apoptosis.