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[Objective] To detect chlorogenic acid,macranthoidin B and dipsacoside B in FLOS LONICERAE grown in Guizhou Province by UPLC-PDA-ELSD,and to investigate the quality of FLOS LONICERAE grown in Guizhou Province. [Methods] ACQUITYUPLC BEH C18 chromatographic column( 2. 1 mm × 100 mm,1. 7 μm) was adopted; mobile phase was acetonitrile( A)- 0. 4% acetic acid water( B); gradient elution was as follows: 0- 2 min,7% A; 2- 2. 3 min,7%- 28% A; 2. 3- 10 min,28% A; 10- 10. 5 min,28%- 7% A; gradient flow rate was as follows: 0- 2 min,0. 35 m L / min; 2- 2. 3 min,0. 35- 0. 25 m L / min; 2. 3- 2. 6 min,0. 25- 0. 15 m L / min; 2. 6- 2. 8min,0. 15- 0. 1 m L / min; 2. 8- 4 min,0. 1- 0. 2 m L / min; 4- 4. 3 min,0. 2- 0. 3 m L / min; 4. 3- 4. 5 min,0. 3- 0. 35 m L / min; 4. 5-10. 5 min,0. 35 m L / min; PDA( ultraviolet detector) was used to detect the chlorogenic acid( 330 nm wavelength); ELSD( evaporative light- scattering detector) was used to detected the macranthoidin B and dipsacoside B; temperature of the drift tube was 60 ℃; sprayer was48 ℃; gas pressure was 137. 9 k Pa; and column temperature was 50 ℃. [Results] Chlorogenic acid,macranthoidin B and dipsacoside B showed good linear relationship within the ranges of 0. 304 5- 1. 522 5 μg / m L( R2= 0. 999 4),0. 449- 1. 436 8 μg / m L( R2= 0. 999 3)and 0. 044 6- 0. 223 μg / m L( R2= 0. 999 4),respectively. [Conclusions] This method could accurately detect the contents of chlorogenic acid,macranthoidin B and dipsacoside B in FLOS LONICERAE grown in Guizhou Province,effectively shorten the measurement time and save reagents,so that it was suitable to detect the quality of FLOS LONICERAE grown in Guizhou Province.