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目的探讨酶消化法和组织块法是否影响牙周膜细胞的特性。方法培养牙周膜细胞,检测克隆形成能力及细胞周期分析,Stro-1的表达,实时定量RT-PCR检测。结果酶消化法培养的PDLCs+G2M明显高于组织块法的PDLC;酶消化法培养的PDLC克隆形式能力明显高于组织块法培养的PDLC;酶消化法培养的PDLC Stro-1的表达明显高于组织块法培养的PDLC;酶消化法培养的PDLC成骨标志物的表达明显高于组织块法培养的PDLC。酶消化法培养的牙周膜细胞具有更强的增殖能力和间充质干细胞含量更多,成骨标志物表达更强。结论酶消化法和组织块法影响牙周膜细胞的某些特性。
Objective To investigate whether enzymatic digestion and tissue blockade affect the characteristics of periodontal ligament cells. Methods Cultured periodontal ligament cells, clonogenic capacity and cell cycle analysis, Stro-1 expression, real-time quantitative RT-PCR detection. Results The PDLCs + G2M cultured by enzymatic digestion method were significantly higher than the PDLC of the tissue block method; the ability of PDLC cloning form cultured by enzyme digestion method was significantly higher than PDLC cultured by tissue block method; the expression of PDLC Stro-1 in the enzymatic digestion method was significantly higher The expression of PDLC osteoblasts cultured by enzymatic digestion method was significantly higher than PDLC cultured by tissue block method. The periodontal ligament cells cultured by enzymatic digestion method have stronger proliferation ability and more mesenchymal stem cells, and the expression of osteogenic markers is stronger. Conclusion Enzymatic digestion and tissue block affect some characteristics of periodontal ligament cells.