【摘 要】
：
Clustered regularly interspaced palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) system has been widely used for precise gene editing in plants.
【机 构】
：
CAS Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture,Wuhan Botanical Garden,T
论文部分内容阅读
Clustered regularly interspaced palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) system has been widely used for precise gene editing in plants. However, simultaneous gene editing of multiple homoeoalleles remains challenging, especially in self-incompatible polyploid plants. Here, we simultaneously in-troduced targeted mutations in all three homoeo-alleles of two genes in the self-incompatible allo-hexaploid tall fescue, using both CRISPR/Cas9 and LbCas12a (LbCpf1) systems. Loss-of-function mu-tants of FaPDS exhibited albino leaves, while knockout of FaHSP17.9 resulted in impaired heat resistance in T0 generation of tall fescue. More-over, these mutations were inheritable. Our findings demonstrate the feasibility of generating loss-of-function mutants in T0 generation polyploid per-ennial grasses using CRISPR/Cas systems.
其他文献
大别山北缘主要由佛子岭群和庐镇关群组成,其处于华北和扬子板块结合处,是探究两大板块耦合过程的关键部位。相对于大别造山带高压-超高压单元的丰硕研究成果,大别山北缘相关的研究较为薄弱,迟滞了对大别造山带演化过程的深入探究。本次研究通过对佛子岭群和庐镇关群详细的野外调查、岩相学、热力学评价和年代学研究,较为详细地阐释了大别山北缘基本变质属性和演化过程。研究表明,佛子岭群主要由各类片岩和部分大理岩构成,并展现了一个巨大的宽缓的“背形”。庐镇关群主体为花岗片麻岩,少部分为斜长角闪岩和石榴黑云母二长片麻岩,其与佛
内蒙古林西县双井地区出露一套含暗色包体的花岗质岩体,位于双井片岩和房框子单元花岗质片麻岩接触带,该岩体对厘定古亚洲洋演化阶段、西伯利亚板块和阿穆尔板块最终碰撞缝合时限具有重要意义。本文对该岩体进行了详细的岩相学、岩石地球化学以及锆石U-Pb年代学研究,讨论了该岩体成因、岩浆源区以及研究区造山带地壳演化等问题。岩体中暗色包体和周边花岗岩大部分锆石记录了-285Ma和-270Ma两期年龄,少量锆石记录了-320Ma的较老年龄,表明该区域在晚石炭世就存在岩浆活动,并在-285Ma、-270Ma更为显著,且岩浆活
构造成岩作用一直是控制盆地深层-超深层、前陆冲断带岩石性质的关键因素,尤其是构造逆冲推覆变形及其对岩石的挤压减孔、破裂造缝和流体改造等作用。为了明确库车坳陷东部中-下侏罗统的岩石构造成岩效应和有利储集性岩石分布,本文基于大量露头实测分析、钻井、地震和测井资料对库车河地区中-下侏罗统开展了构造-岩石响应和有利储层分布预测研究。结果表明:喜山晚期南天山隆起强烈的逆冲推覆活动产生巨大的侧向构造挤压,库车河地区最大有效古应力为60-120MPa,迪北地区为90-120Mpa,吐格尔明地区为60-90MPa。构造挤
识别并研究扬子板块古元古代的岩浆-变质-沉积事件,是探讨扬子板块古元古代构造演化的基础,也是重建该陆块在Columbia超大陆中位置的前提。新发现的金盆杂岩体为进一步揭示扬子板块古元古代岩浆事件和造山过程提供了新的制约信息。锆石U-Pb定年结果表明,金盆片麻状二长花岗岩、奥长花岗岩和基性岩脉的形成年龄分别为-2478Ma、-2049Ma和-1999Ma。奥长花岗岩具有高的SiO2(71.62%-76.92%)、Na2O(4.58%-6.47%)含量,低的K2O(
Polycystic ovary syndrome (PCOS) is a heterogeneous disorder with evidence of polygenetic components,and obesity may be a risk factor for hyperandrogenism.Previ
穿地壳岩浆系统理念的提出为认知岩浆岩的形成机制提供了一个新的窗口。在穿地壳岩浆系统内,深层次岩浆储库结晶的矿物(例如辉石、角闪石、斜长石等)可通过通道向上运移至浅层次的岩浆储库,该过程可导致这些矿物的熔蚀、流体交代或再生长。上述矿物属于再循环晶,它们保留的成分环带信息可忠实地记录岩浆环境的变化。本文以华北克拉通东南缘胶北早白垩世丛家岩体(-127Ma)内产出的闪斜-拉辉煌斑岩为主要研究对象。这些钙碱性煌斑岩中常见不同结构和成分特征的单斜辉石再循环晶,它们保存了多级岩浆储库的相关信息。丛家煌斑岩包括一系列中
Processing of mature transfer RNAs(tRNAs)produces complex populations of tRNA-derived fragments(tRFs).Emerging evidence shows that tRFs have important functions in bacteria,animals,and plants.Here,we review recent advances in understanding plant tRFs,focu
Toll-like receptor 3(TLR3)is a member of the TLR family,mediating the transcriptional induction of type I interferons(IFNs),proinflammatory cytokines,and chemokines,thereby collectively establishing an antiviral host response.Studies have shown that unlik
This paper aims to the debate on the nexus between informal economies and the environment by investigating the long-term dynamic impacts of China's informal ec
The ubiquitous volatile linalool is metabolized in plants to nonvolatile derivatives.We studied Nicotiana attenuata plants which naturally vary in(S)-(+)-linalool contents,and lines engineered to produce either(R)-(-)-or(S)-(+)-linalool.Only(S)-(+)-linalo