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目的:研究新城疫病毒NDV7793对人结肠癌细胞的体外杀伤作用,为结肠癌的生物疗法奠定基础。方法:通过蚀斑实验纯化病毒并测定纯化的NDV7793株的感染力;用乳酸脱氢酶微量释放法测定纯化病毒对人LoVo和Ls174t结肠癌细胞株的杀伤作用并且通过血凝实验测定病毒在不同细胞中的增殖力。结果:NDV7793在感染细胞96h后出现直径约为0.5mm左右的空斑,PFU为1.25×107个/ml,为弱毒株;NDV7793对LoVo和Ls174t人结肠癌细胞株有明显的杀伤作用,而且杀伤作用的强度与病毒作用的时间和病毒的浓度呈正相关的关系;NDV7793可以在肠癌细胞中生长复制,该病毒株在人结肠癌细胞株LoVo的复制能力强于Ls174t。结论:NDV7793具有较强的选择性杀伤人结肠癌细胞的作用,且为弱毒株,这株病毒具备肿瘤生物治疗的潜能。
Objective: To study the in vitro killing effect of Newcastle disease virus NDV7793 on human colon cancer cells and lay the foundation for the biological therapy of colon cancer. Methods: The virus was purified by plaque assay and the infectivity of the purified NDV7793 strain was determined. The killing effect of the purified virus on human LoVo and Ls174t colon cancer cell lines was determined by lactate dehydrogenase microdeletion method and the virus was determined by hemagglutination assay Proliferation in cells. Results: NDV7793 appeared plaques with a diameter of about 0.5mm after infection for 96h, with a PFU of 1.25 × 107 cells / ml, which was a virulent strain. NDV7793 had obvious killing effect on LoVo and Ls174t human colon carcinoma cell lines, The intensity of action was positively correlated with the time of virus and the concentration of virus. NDV7793 could grow and replicate in colon cancer cells. The replication of this strain in human colon cancer cell line LoVo was stronger than that of Ls174t. Conclusion: NDV7793 has a strong selective killing effect on human colon cancer cells and is a virulent strain that has the potential of tumor biotherapy.