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目的喉癌患者穿通支原体的检出,及其淋巴转移和肿瘤组织NF-κBp65表达分析,试图探讨穿通支原体感染与喉癌的发生、淋巴转移及其肿瘤组织NF-κBp65的表达之间相关性。方法采用分离培养和巢式聚合酶链反应检测93例喉癌和40例对照组中穿通支原体的存在,阳性标本进一步电镜确认,并对阳性和阴性患者进行淋巴转移及其肿瘤组织NF-κBp65表达分析。结果喉癌患者穿通支原体感染率明显高于非肿瘤组(P<0.05),穿通支原体阳性组患者淋巴结转移率明显高于穿通支原体阴性组(P<0.05),穿通支原体阳性组NF-κBp65表达显著高于穿通支原体阴性组(P<0.05)。电镜观察见穿通支原体阳性肿瘤组织中3层膜结构的穿通支原体。结论穿通支原体感染与喉癌的发生、淋巴转移及其肿瘤组织NF-κBp65表达呈正相关。
Objective To investigate the detection of mycoplasma penetrating Mycobacterium tuberculosis and its lymphatic metastasis and the expression of NF-κBp65 in the tumor tissue of patients with laryngeal cancer. The aim of this study was to explore the correlation between the infection of Mycoplasma penetrans and the occurrence of laryngeal carcinoma, lymphatic metastasis and the expression of NF-κBp65 in tumor tissues. Methods The mycoplasma was isolated from 93 cases of laryngeal carcinoma and 40 cases of control group by means of isolation and culture and nested polymerase chain reaction. The positive specimens were confirmed by electron microscopy and the positive and negative expressions of NF-κBp65 analysis. Results The infection rate of mycoplasma in laryngeal cancer patients was significantly higher than that in non-tumor patients (P <0.05). The rate of lymph node metastasis was significantly higher in patients with penetrating mycoplasma-positive than that in patients with penetrating mycoplasma (P <0.05). The expression of NF-κBp65 Which was higher than that in the negative group (P <0.05). Electron microscopy see through mycoplasma positive tumor tissue membrane structure of the three through-mycoplasma. Conclusion The infection of Mycoplasma penetrans has a positive correlation with the occurrence of laryngeal carcinoma, lymphatic metastasis and the expression of NF-κBp65 in tumor tissues.