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采用庆大霉素产生菌—绛红小单孢菌(M.purpurea)在培养36h时添加15μg/ml的青霉素,对培养至48h对数生长期的菌体进行紫外线照射+亚硝基胍复合处理,置培养箱中37℃培养5～8d待长出菌落后再用紫外线照射叠加处理,获得一高产菌株N-14。试管发酵生测效价1913u/ml(对照625u/ml);摇瓶发酵培养120h达2178(u/ml),比对照(1243u/ml)提高75.2%;并进行了5L发酵罐发酵培养,发酵过程中测定了发酵液还原糖、总糖、氨基氮、pH、菌体浓度等代谢参数,探索了适合N-14的培养条件,连续5批次生测效价平均1967u/ml,较对照1171u/ml提高了68%,效果明显。
The gentamicin-producing strain, M.purpurea, was added with 15μg / ml penicillin at 36h after culture, and the cells cultured in the logarithmic growth phase at 48h were irradiated with UV light and combined with nitrosoguanidine Treatment, set the incubator at 37 ℃ for 5 ~ 8d to be out of the long after the colonies and then treated with ultraviolet irradiation superposition to obtain a high yield strain N-14. The titer of test tube fermentation was 1913u / ml (control 625u / ml); the shake flask culture reached 2178 (u / ml) at 120h, which was 75.2% higher than the control (1243u / ml); The metabolic parameters of reducing sugar, total sugar, amino nitrogen, pH, and cell density of the fermentation broth were determined, and the culture conditions suitable for N-14 were explored. The titer of continuous batch of 5 batches was 1967u / ml on average, / ml increased by 68%, the effect is obvious.