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目的:考查番茄红素(lycopene)对原代培养大鼠皮层神经细胞低糖氧损伤的保护作用,并对其可能的线粒体作用进行分析。方法:原代培养大鼠皮层神经细胞于培养d 7分为正常组、低糖无氧损伤组(95%N2/5%CO2饱和的低糖DMEM培养基)、番茄红素低、中、高剂量组(10-7,10-6和10-5mol·L-1)。番茄红素在低糖氧损伤的同时加入培养液。观察细胞坏死与凋亡、ATP水平、线粒体总量、线粒体膜电位及线粒体呼吸功能。结果:番茄红素能明显减少低糖氧损伤导致的神经细胞坏死与凋亡,提高细胞内ATP水平和线粒体总量,提高线粒体膜电位水平,并明显改善线粒体呼吸功能。结论:番茄红素对原代培养大鼠皮层神经细胞低糖氧损伤具有明显保护作用,这一作用与其保护和改善线粒体呼吸功能和能量代谢密切相关。
OBJECTIVE: To investigate the protective effect of lycopene on hypoxia-induced oxygenated injury of primary cultured rat cortical neurons and to analyze its possible mitochondrial function. Methods: Primary cultured rat cortical neurons were divided into normal group, low glucose anoxic group (low glucose DMEM medium with 95% N2 / 5% CO2 saturation), low, medium and high dose lycopene (10-7, 10-6 and 10-5 mol·L-1). Lycopene is added to the culture broth at the same time as low oxygen sugar damage. Cell necrosis and apoptosis, ATP level, total amount of mitochondria, mitochondrial membrane potential and mitochondrial respiratory function were observed. Results: Lycopene could obviously reduce the necrosis and apoptosis of nerve cells induced by hypoglycemia, increase the intracellular ATP level and total amount of mitochondria, increase the level of mitochondrial membrane potential and significantly improve mitochondrial respiratory function. CONCLUSION: Lycopene has a significant protective effect on hypoxia-induced oxygenated injury of primary cultured rat cortical neurons, which is closely related to its protection and improvement of mitochondrial respiratory function and energy metabolism.