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目的:建立妇乐安颗粒的质量控制方法。方法:采用TLC法对方中柴胡、金银花和当归进行定性鉴别;采用HPLC法对处方中栀子苷的含量进行定量检测。结果:薄层色谱鉴别方法专属性强,重复性好,阴性对照无干扰;栀子苷线性范围为0.03～0.53μg,r=0.999 7,平均回收率99.69%,RSD为0.6%。结论:薄层色谱方法简便快捷,结果可靠;含量测定方法操作简便、灵敏度高、结果准确,可用于妇乐安颗粒中栀子苷的测定。本试验为妇乐安颗粒的质量控制提供了依据。
Objective: To establish the quality control method of Fulean Granules. METHODS: The qualitative identification of Bupleurum chinense, honeysuckle and Angelica sinensis was performed by TLC method. The contents of jasminoidin in the prescription were detected by HPLC. RESULTS: The TLC method was characterized by strong specificity, good reproducibility, and no interference with negative controls. The linear range of geniposide was 0.03-0.53 μg, r=0.999, the average recovery rate was 99.69%, and the RSD was 0.6%. Conclusion: The TLC method is simple and rapid, and the result is reliable. The content determination method is simple, sensitive and accurate. It can be used for the determination of geniposide in Fulean Granules. This test provides the basis for the quality control of Fu Lean particles.