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目的探讨影响肝癌生物学行为及预后的重要因素和寻求肝癌基因治疗的有效靶点。方法运用免疫组化方法检测Ki67、nm23H1、cMet及MT1MMP蛋白在93例肝细胞癌组织中的表达水平,并判断其与肝癌细胞恶性生物学行为及患者预后的关系。结果Ki67、nm23H1、cMet及MT1MMP蛋白表达水平与肝癌患者预后均具有显著相关性(P均<0.01);nm23H1的表达与肿瘤远处转移之间呈负相关关系(P=0.041);cMet、MT1MMP的表达与肝癌瘤旁浸润及远处转移之间均呈正相关关系(P<0.05)。结论nm23H1基因的失活或突变,伴随cMet以及MT1MMP蛋白的异常表达可能是导致肝癌浸润、转移的关键因素。
Objective To explore the important factors affecting the biological behavior and prognosis of liver cancer and find effective targets for gene therapy of liver cancer. Methods Immunohistochemistry was used to detect the expression of Ki67, nm23H1, cMet and MT1 MMP in 93 cases of hepatocellular carcinoma. The relationship between the expression of Ki67, nm23H1, cMet and MT1 MMP protein and the malignant biological behavior of hepatocellular carcinoma and its prognosis was also determined. Results The expressions of Ki67, nm23H1, cMet and MT1MMP proteins were significantly correlated with the prognosis of patients with liver cancer (P<0.01). The expression of nm23H1 was negatively correlated with the distant metastasis of tumors (P=0.041); cMet, MT1MMP There was a positive correlation between the expression of hepatocellular carcinoma and paratumor invasion and distant metastasis (P<0.05). Conclusion The inactivation or mutation of nm23H1 gene, along with the abnormal expression of cMet and MT1 MMP protein may be the key factors leading to infiltration and metastasis of liver cancer.