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一九八三年美国哈佛医学院的科学家第一个分离、纯化了人载脂蛋白A-Ⅰ基因,其后又对它的核苷酸顺序进行了分析。整个apoA-Ⅰ基因有三个插入顺序;apoA-Ⅰ的mRNA初级翻译产物由267个氨基酸组成,其中包括成熟的apoA-Ⅰ243个氨基酸和前原片段(preprosegment)的24个氨基酸。其3′末端非编码区由55个核苷酸组成,其Poly(A)信号(AAUAAA)位于终止密码下游36个碱基对处。apoA-Ⅰ基因与另一个载脂蛋白apoC-Ⅲ基因紧密连锁,apoC-Ⅲ基因位于apoA-Ⅰ基因3′末端下游大约2.6kb处,并且可以同时进行转录。Shoulders和Sharpe也分离纯化了apoA-Ⅰ的cDNA。随后确定了apoA-Ⅰ基因在染色体上的定位。
In 1983, scientists at Harvard Medical School in the United States first isolated and purified human apolipoprotein A-Ⅰ gene, and then analyzed its nucleotide sequence. The entire apoA-I gene has three insertions; the apoA-I mRNA primary translation product consists of 267 amino acids, including 243 amino acids of the mature apoA-I and 24 amino acids of the preprosegment. Its 3 ’end non-coding region consists of 55 nucleotides, and its Poly (A) signal (AAUAAA) is located 36 base pairs downstream of the stop codon. The apoA-I gene is tightly linked to another apolipoprotein apoC-III gene. The apoC-III gene is located approximately 2.6 kb downstream of the 3 ’end of the apoA-I gene and can be transcribed simultaneously. Shoulders and Sharpe also isolated and purified apoA-Ⅰ cDNA. Subsequently, the apoA-I gene was located on the chromosome.