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目的对中国汉族人群的apoB VNTR等位基因进行分布频率、序列结构等多态性分析,并通过分子克隆技术制备apoB VNTR等位基因分型标准物。方法通过PCR扩增并结合电泳、测序等方法分析人群apoB VNTR等位基因的多态性,并将该人群中得到的所有类型等位基因片段插入pUC重组质粒,经转染、扩大培养、扩增及再鉴定后,制备出apoB VNTR等位基因分型标准物。结果所检测人群中共检测出16种apoB VNTR等位基因,发现核心序列存在变异体,并成功制备出分型标准物。结论对apoB VNTR的多态性分析应结合等位基因的分布频率与核心序列的结构,所制备的apoB VNTR等位基因分型标准物使apoB VNTR电泳法分型更加准确。
Objective To analyze the distribution frequency and sequence structure of apoB VNTR alleles in Chinese Han population and to prepare apoB VNTR allelic typing standard by molecular cloning technology. Methods The polymorphism of the apoB VNTR allele was analyzed by PCR amplification and electrophoresis, sequencing and so on. All the allelic fragments obtained from this population were inserted into pUC recombinant plasmids and transfected, expanded and expanded ApoB VNTR allelic typing standards were prepared after increasing and re-qualification. Results A total of 16 apoB VNTR alleles were detected in the tested population. The core sequence variants were found and the typing standards were successfully prepared. Conclusion Polymorphism analysis of apoB VNTR should be based on the distribution frequency of alleles and the structure of the core sequence. The apoB VNTR allelic typing standard makes the apoB VNTR electrophoresis more accurate.