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目的探讨食管癌Hp-L型(即球形:Hp)感染与肿瘤血管形成的相关性及Hp-L型对食管癌生长、侵袭和转移等生物学行为的影响。方法应用革兰染色、电镜和免疫组化ABC法,检测98例食管癌和30例对照组(食管癌切缘正常组织)的Hp-L型;用免疫组化SP法检测上述组织的微血管密度(MVD)值和血管内皮生长因子(VEGF)、p53表达,分析Hp-L型与MVD、VEGF、p53表达以及临床病理因素的关系。结果 食管癌组的Hp-L型阳性率为60.2％。电镜下组织中的Hp-L型有两种类型,可位于癌细胞间或癌细胞胞浆。食管癌组的Hp-L型阳性率、MVD值以及VEGF、p53表达阳性率均高于对照组(P<0.005～0.001),食管癌组中Hp-L型阳性组的MVD值、VEGF和p53表达阳性率也高于其Hp-L型阴性组(P<0.005～0.001)。Hp-L型阳性与MVD呈正相关(r=0.46,P<0.01),与VEGF、p53表达呈正相关(r=0.31,P<0.01)。食管癌组的Hp-L型阳性数与癌细胞的血管侵袭、侵袭深度、食管旁淋巴结转移及远处淋巴结转移相关,而与肿瘤大小无关。结论 Hp-L型与食管癌的血管形成密切相关,是影响食管癌生长、侵袭和转移的重要因素之一。
Objective To investigate the relationship between Hp-L (esophageal Hp) infection and tumor angiogenesis and the effect of Hp-L on the biological behavior of esophageal cancer such as growth, invasion and metastasis. Methods The Hp-L of 98 cases of esophageal cancer and 30 cases of control group (normal tissue of esophageal cancer) were detected by Gram stain, electron microscope and immunohistochemical ABC method. The microvessel density (MVD), vascular endothelial growth factor (VEGF) and p53 expression, and to analyze the relationship between Hp-L and MVD, VEGF, p53 expression and clinical pathological factors. Results The positive rate of Hp-L in esophageal cancer group was 60.2%. There are two types of Hp-L in the tissue under the electron microscope, which can be located between the cancer cells or the cytoplasm of cancer cells. The positive rate of Hp-L, MVD and the positive expression rate of VEGF and p53 in esophageal cancer group were higher than those in control group (P <0.005-0.001). The MVD value, VEGF and p53 in Hp-L positive group The positive expression rate was also higher than that of Hp-L negative group (P <0.005 ~ 0.001). The positive expression of Hp-L was positively correlated with MVD (r = 0.46, P <0.01), and positively correlated with the expression of VEGF and p53 (r = 0.31, P <0.01). The positive number of Hp-L in esophageal cancer group was related to the invasion, invasion depth, lymph node metastasis and distant lymph node metastasis of cancer cells, but not with tumor size. Conclusion Hp-L type is closely related to the angiogenesis of esophageal cancer and is one of the important factors that affect the growth, invasion and metastasis of esophageal cancer.