在海拔5200m的青藏高原唐古拉山，实地野外取实验动物的组织器官，观察其超微结构的形态变化，对于高山病基础研究有一定帮助。然而高海拔地区高寒缺氧、昼夜温差大，野外现场条件差，设备简陋。且电镜取材技术必须熟练精细，标本固定液和制片技术要求较高，又无前人经验借鉴， In the Tanggula plateau of Qinghai-Tibet Plateau at 5200m above sea level, the tissues and organs of experimental animals were taken in the field and the morphological changes of the ultrastructure were observed. It is helpful for the basic research of alpine diseases. However, the high-altitude areas are cold and hypoxia, with large temperature difference between day and night, poor field conditions and poor facilities. And electron microscopy drawing technology must be sophisticated, specimen fixing solution and the preparation of high technical requirements, without prior experience,