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目的了解浙江省衢州市肠出血性大肠埃希菌O157∶H7人的感染、食品污染以及动物和媒介昆虫带菌情况。方法在流行季节采集腹泻患者、动物粪便以及各类食品、苍蝇等样本,MEC肉汤增菌后,用特异性免疫磁珠集菌,以科玛嘉显色平板分离,纯化的菌株进行生化鉴定、血清分型及毒力基因检测。结果 2000-2009年,共检测样本12 292份,检出EHEC O157∶H7 19株,总检出率为0.15%。其中4种宿主动物粪便样本中检出18株,检出率分别为羊2.14%、牛1.29%、猪0.45%、鸭0.19%;食品中从生猪肉中检出1株,检出率0.17%;腹泻患者和苍蝇样本中均未检出。经毒力基因检测,其中16株stx2+hly+eaeA阳性,1株hly+eaeA阳性,其余2株不带毒力基因,带毒率89.47%。结论浙江省衢州市部分动物中产毒的EHEC O157∶H7带菌率较高,表明该地区存在暴发或流行的潜在危险,应加强综合监测。
Objective To understand the infection of enterohemorrhagic Escherichia coli O157: H7 in Quzhou City of Zhejiang Province, the contamination of foodstuffs and the carriage of animal and vector insects. Methods Diarrhea patients, animal feces, flies and other samples were collected in the epidemic season. After enrichment with MEC broth, the bacteria were collected by specific immunomagnetic beads and biochemically identified by the strains isolated and purified by Kema Jia chromogenic plate , Serotyping and virulence gene testing. Results From 2000 to 2009, a total of 12 292 samples were tested, and 19 strains of EHEC O157: H7 were detected with a total detection rate of 0.15%. Among them, 18 strains were detected in the stool samples of 4 kinds of host animals, the detection rates were 2.14%, 1.29%, 0.45% and 0.19% respectively. One strain was detected in raw pork from food, the detection rate was 0.17% Neither diarrhea nor flies samples were detected. The virulence gene test, of which 16 stx2 + hly + eaeA positive, 1 hly + eaeA positive, the remaining two strains without virulence genes, with a virulence rate of 89.47%. Conclusion The higher incidence of toxigenic EHEC O157:H7 in some animals in Quzhou City of Zhejiang Province indicates that there is a potential risk of outbreak or epidemic in this area and comprehensive monitoring should be strengthened.