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小麦萌发时,种子胚乳总蛋白含量逐渐下降,其中由于提供萌发所需氮化物球蛋白、醇溶蛋白和谷蛋白逐渐减少,合成数种启动种子萌发所需酶蛋白等的清蛋白逐渐增加。胚中蛋白质各组分随种子萌发过程均逐渐增加。渗透胁迫下,萌发种子胚乳清蛋白和胚中蛋白质各组分的增加,以及胚乳中球蛋白、醇溶蛋白和谷蛋白的降解均受到抑制。因此,种子萌发与其蛋白质变化密切相关。Ca处理在萌发后期才对种子蛋白质变化起促进作用,GA和Ca+GA处理在萌发后一直起促进作用。本实验结果表明,Ca/GA处理种子通过对萌发中蛋白质的调节,可减缓渗透胁迫对萌发造成的不良影响。对胚乳和胚中蛋白质变化的调节效果均是GA和Ca+GA处理优于Ca处理。
The total protein content of seed endosperm decreased gradually during wheat germination, in which the number of albumin required for synthesis of several enzyme proteins required for seed germination was gradually increased due to the gradual decrease of gliadin, gliadin and gluten required for germination. Embryo protein components with the seed germination process are gradually increased. Under osmotic stress, the increase of protein components in germinated seed embryos and embryos, and the degradation of globulin, gliadin and gluten in endosperm were inhibited. Therefore, the seed germination and its protein changes are closely related. Ca treatment promoted the changes of seed protein at the late germination stage, and GA and Ca + GA treatments had a promoting effect after germination. The experimental results show that Ca / GA treatment of seeds through the regulation of the protein in germination, can slow down the osmotic stress on germination adverse effects. The effects of GA and Ca + GA treatment on protein changes in endosperm and embryo were better than Ca treatment.