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目的探讨人淋巴管胚胎期的发生和发育及叉头框C2(FOXC2)的表达。方法用淋巴管内皮透明质酸受体1(LYVE-1)作为淋巴管的标记物,应用免疫组织化学SP染色及免疫荧光双标染色的方法,检测FOXC2和LYVE-1在妊娠5～11周的85例人胚胎标本淋巴管的表达及淋巴管的发生发育情况。结果妊娠7周后的胎儿颈部及胸部的淋巴管出现LYVE-1表达。在妊娠大约10周时,胎儿肠系膜间淋巴管内皮细胞开始出现LYVE-1表达。FOXC2的表达早于LYVE-1,于妊娠第6周开始在中胚层间充质明显表达。FOXC2不仅表达于淋巴管,并广泛分布于椎体、心血管等部位。妊娠11周后的胎儿淋巴管内皮细胞中仍能见到FOXC2和LYVE-1的表达。结论在妊娠7～8周间(人胚胎发育35～42d),淋巴管开始形成,FOXC2和LYVE-1与淋巴管的发生和发育相关。
Objective To investigate the occurrence and development of human lymphatic vessel embryogenesis and the expression of forkhead box C2 (FOXC2). Methods Lymph tube endothelial hyaluronic acid receptor 1 (LYVE-1) was used as a marker of lymphatic vessels. Immunohistochemical staining and immunofluorescence staining were used to detect the expression of FOXC2 and LYVE-1 in the 5th to 11th week of gestation 85 cases of human embryo lymphatic vessel expression and lymphatic vessel development. Results The expression of LYVE-1 in the lymphatic vessels of fetal neck and chest after 7 weeks gestation. At about 10 weeks of pregnancy, LYVE-1 expression begins to develop in mesenteric lymphatic endothelial cells of the fetus. The expression of FOXC2 was earlier than that of LYVE-1, and the expression of FOXC2 in mesoderm was significant at the 6th week of gestation. FOXC2 is not only expressed in the lymphatic vessels, and widely distributed in the vertebral body, cardiovascular and other parts. FOXC2 and LYVE-1 expression can still be seen in fetal lymphatic endothelial cells 11 weeks after gestation. Conclusions Lymphatic vessels begin to form in the 7th to 8th week of pregnancy (35-42 days of human embryo development), and FOXC2 and LYVE-1 are related to the occurrence and development of lymphatic vessels.