论文部分内容阅读
为了能对固定化青霉素G酰化酶进行X射线微区分析 ,筛选了能捕捉酶活的合适的底物与捕捉剂的体系 ,青霉素G钠作为底物 ,FeCl3 作为捕捉剂 ,底物经固定化青霉素G酰化酶水解产生苯乙酸 ,后者与捕捉剂反应生成沉淀 ,可以确定固定化青霉素G酰化酶的催化活性部位 ;还对捕捉剂与底物、固定化青霉素G酰化酶与载体以及载体与底物之间的相互作用进行了研究 ,找到了可用于对固定化青霉素G酰化酶活性进行X射线微区定位的捕捉剂.
In order to perform X-ray microanalysis on immobilized penicillin G acylase, we screened a system that can capture the appropriate substrate and capture agent for enzyme activity. Penicillin G sodium was used as the substrate and FeCl3 was used as the capture reagent. The substrate was immobilized The penicillin G acylase hydrolyzes to give phenylacetic acid, which reacts with the capture reagent to form a precipitate that determines the catalytically active site of the immobilized penicillin G acylase. The capture agent and substrate, immobilized penicillin G acylase and The interaction between the carrier and the support and the substrate was investigated and a capture agent found to be useful for X-ray micro-localization of immobilized penicillin G acylase activity was found.