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采用菌丝生长速率法测定了河北省马铃薯主产区的122株早疫病菌Alternaria alternata对咯菌腈的敏感性,并测定了紫外诱导的15个抗咯菌腈突变菌株及其3个敏感亲本菌株对咯菌腈、异菌脲、腐霉利、苯醚甲环唑、吡唑醚菌酯的敏感性。田间马铃薯早疫病菌群体对咯菌腈保持高度敏感,122株早疫病菌对咯菌腈的EC50值在0.0025～0.0857μg/mL之间,平均值为0.0404±0.0171μg/mL,相差34.87倍,呈近似正态分布,可作为早疫病菌对咯菌腈的敏感基线。此外,咯菌腈与吡唑醚菌酯和苯醚甲环唑之间无交互抗性关系,而与异菌脲和腐霉利之间存在正交互抗性关系。在田间药效试验中,50%咯菌腈WP施药量为105、115 g/hm2时,对马铃薯早疫病防效达80%以上,显著高于常规药剂80%代森锰锌WP 1350g/hm2和25%丙环唑EC 37.5g/hm2的防效。表明咯菌腈可作为吡唑醚菌酯和苯醚甲环唑的替代药剂用于马铃薯早疫病的防治。
The mycelial growth rate method was used to determine the susceptibility of Alternaria alternata to fludioxonil in 122 main producing areas of potato in Hebei province. Fifteen mutagens of fluconazole induced by UV and three sensitive parents Susceptibility to fludioxonil, iprodione, procymidone, difenoconazole, pyraclostrobin. The field-susceptible A. solani isolates were highly susceptible to fludioxonil. EC50 values of 122 isolates of flumatidone were between 0.0025-0.0857 μg / mL, with an average of 0.0404 ± 0.0171 μg / mL, a difference of 34.87 folds, It showed an approximately normal distribution and could be used as a sensitive baseline of fludioxonil for Alternaria solani. In addition, there was no cross-resistance between fludioxonil and pyraclostrobin and difenoconazole, but there was an orthogonal cross-resistance relationship between iprodione and pyrimethamine. In the field efficacy test, 50% fludioxonstans WP was 105, 115 g / hm2, the control effect on potato early blight was more than 80%, which was significantly higher than 80% of the conventional pharmaceutical mancozeb WP 1350g / hm2 and 25% propiconazole EC 37.5g / hm2 control effect. Fludioxonil was used as an alternative to pyraclostrobin and difenoconazole for the control of potato early blight.