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目的对中国C群脑膜炎奈瑟菌分离株进行脉冲场凝胶电泳(PFGE)分析,了解安徽省C群流行性脑脊髓膜炎(流脑)暴发菌株及国内C群脑膜炎奈瑟菌菌株的分子流行病学特征。方法212株C群脑膜炎奈瑟菌菌株分离自流脑患者、密切接触者和健康人群鼻咽部,包括安徽省C群流脑菌株48株,其中38株与C群流脑暴发相关。脉冲场凝胶电泳选用NheⅠ限制性内切酶,聚类分析选用BioNumerics软件。结果212株C群脑膜炎奈瑟菌菌株共分为43个PFGE带型,命名为AHl～AH43,AH1是中国C群脑膜炎奈瑟菌主要的带型,占69.3%(n=147),分布于11个省市。安徽省48株C群菌株共分为3个PFGE型别(AH1、AH2、AH3),45株(93.8%)为AH1型。安徽省38株与C群流脑暴发相关的菌株中,37株(97.4%)为AH1型。全国53株流脑患者分离菌株中,AH1型菌株占67.9%(36/53);121株流脑病例密切接触者分离菌株中。AH1型占71.9%(87/121),38株健康人群鼻咽部分离菌株中,AH1型占63.2%(24/38)。结论AH1型C群脑膜炎奈瑟菌是中国目前C群流脑流行的主要克隆群,安徽省C群流脑暴发是由AH1型C群脑膜炎奈瑟菌引起的,已在全国呈扩散流行的趋势。
Objective To analyze the epidemic encephalitis group C of Chinese meningitis serogroup M. meningitidis in Anhui Province by pulsed-field gel electrophoresis (PFGE) Molecular epidemiology. Methods A total of 212 strains of N. meningitidis from Group C were isolated from nasopharynx of meningococcal patients, close contacts and healthy people, including 48 meningitis group C in Anhui Province. Among them, 38 strains were associated with outbreaks of group C meningitis. Pulsed-field gel electrophoresis selection Nhe Ⅰ restriction enzyme, clustering analysis using BioNumerics software. Results 212 ND strains of N. meningitidis were divided into 43 PFGE bands, named AH1 ~ AH43. AH1 was the main band type of Neisseria meningitidis group C in China, accounting for 69.3% (n = 147) Distributed in 11 provinces. 48 strains of C in Anhui Province were divided into three PFGE types (AH1, AH2, AH3), 45 (93.8%) AH1 type. Of the 38 isolates in Anhui Province that were associated with outbreaks of Group C meningitis, 37 (97.4%) were AH1. Among the 53 isolates isolated from China, 67 (36.9%) were AH1 and 121 were closely related to meningitis. AH1 type accounted for 71.9% (87/121), 38 healthy people nasopharyngeal isolates, AH1 type accounted for 63.2% (24/38). Conclusion Neisseria meningitidis serogroup C (AH1) is the most common clonal group of meningitidis in China. The outbreak of meningitis group C in Anhui Province is caused by Neisseria AH1 group C. meningitidis and has spread throughout the country the trend of.