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目的:采用MTT法对胃癌患者的癌细胞及外周血淋巴细胞进行抗癌药的体外药敏实验,找出两者之间的相关性,探求指导非手术胃癌患者化疗用药个体化的新途径。方法:在采集手术标本时抽取该患者外周血,分别用LFAM方案的用药(四氢叶酸钙,5-氟尿嘧啶,阿霉素,丝裂霉素)进行体外实验,MTT法检测36例胃癌组织细胞及其外周血淋巴细胞的抑制率,利用SPSS10.0软件计算两者之间的相关性。结果:36例胃癌患者的新鲜癌组织细胞与外周血淋巴细胞的抑制率有很好的相关性。结论:对失去手术机会或病灶很小不能取材的胃癌患者外周血淋巴细胞可代替癌细胞做MTT体外药敏检测。
OBJECTIVE: To investigate the drug susceptibility of cancer cells and peripheral blood lymphocytes in patients with gastric cancer by MTT assay and to find out the correlation between the two, and to explore a new way to guide the individualization of chemotherapeutic drugs in patients with non-surgical gastric cancer. Methods: Peripheral blood samples were collected from the patients during operation. The LFAM regimen (calcium folinate, 5-fluorouracil, doxorubicin and mitomycin) And its peripheral blood lymphocyte inhibition rate, the use of SPSS10.0 software to calculate the correlation between the two. Results: There was a good correlation between the fresh cancer cells and the peripheral blood lymphocytes in 36 gastric cancer patients. Conclusion: Peripheral blood lymphocytes can replace MTT in vitro MTT assay in patients with gastric cancer who have lost the chance of surgery or have small lesions.