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目的了解无偿献血者隐匿性乙型肝炎病毒(HBV)感染的血清学状况,减少输血传播HBV感染风险。方法收集乙肝表面抗原(HBsAg)阴性无偿献血者可疑HBV感染血液2175份,所有血液标本均通过ELISA法检测血清HBV相关标志物,抗-HBC阳性血清再通过PCR检测HBV DNA。结果 2175例HBsAg阴性标本共有抗-HBC阳性标本700例(32.2%),单独抗-HBC阳性者有153例,抗-HBC和抗-HBs同时阳性有260例,其中有32.0%(224/700)为抗-HBs弱阳性,有46.1%(323/700)为抗-HBs强阳性。抗-HBC阳性标本中HBV DNA阳性率为4.7%(33/700)。HBV DNA阳性标本中有57.6%(19/33)为抗-HBs阴性,有39.4%(13/33)为抗-HBs弱阳性,有3.0%(1/33)为抗-HBs强阳性。结论无偿献血者存在隐匿性HBV感染,有必要重视无偿献血者初筛HBsAg阴性人群的进一步血清学检测。
Objective To understand the serological status of occult hepatitis B virus (HBV) infection in unpaid blood donors and to reduce the risk of transfusion transmitted HBV infection. Methods A total of 2175 blood samples were collected from suspected uninfected donors with negative HBsAg. Serum HBV-related markers were detected by ELISA in all blood samples, and HBV DNA was detected by PCR. Results Among the 2175 HBsAg negative specimens, there were 700 anti-HBC positive specimens (32.2%), 153 anti-HBC positive specimens alone, 260 positive specimens of anti-HBC and anti-HBs, of which 32.0% ) Were weakly positive for anti-HBs, 46.1% (323/700) were strongly positive for anti-HBs. The positive rate of HBV DNA in anti-HBC positive specimens was 4.7% (33/700). Among the HBV DNA positive samples, 57.6% (19/33) were negative for anti-HBs, 39.4% (13/33) were weakly positive for anti-HBs and 3.0% (1/33) were strongly positive for anti-HBs. Conclusion There are occult HBV infection in the blood of unpaid blood donors. It is necessary to pay attention to the further serological detection of HBsAg-negative population of unprotected blood donors.