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目的将桑白皮蜜炙前后水提液的HPLC指纹图谱进行对比,研究桑白皮蜜炙前后水提液的差异。方法采用Waters 2965高效液相色谱仪,Waters 2996二极管阵列检测器,色谱柱Thermo ODS-2 HYPERSIL(250 mm×4.6 mm,5μm),色谱条件:乙腈-0.4%冰醋酸水溶液梯度洗脱;柱温30℃;流速1 ml·min-1;检测波长为340 nm;进样量10μl。结果桑白皮蜜炙前后指纹图谱保留时间变化不大,RSD均小于3%;蜜炙前后化学成分的峰面积有所改变,但趋势并不相同,其中东莨菪内酯的变化呈下降趋势。结论该文为进一步研究桑白皮蜜炙前后化学成分的变化奠定了基础。
Objective To compare the HPLC fingerprints of water extract of Cortex Mori before and after Sunburn, to study the differences of water extract before and after Sunburn. Methods Waters 2965 high performance liquid chromatograph, Waters 2996 diode array detector, column Thermo ODS-2 HYPERSIL (250 mm × 4.6 mm, 5 μm) were used. The chromatographic conditions were as follows: acetonitrile-0.4% glacial acetic acid gradient elution; 30 ℃; flow rate 1 ml · min-1; detection wavelength was 340 nm; injection volume 10μl. Results Before and after Sunburn, the retention time of fingerprints did not change much, RSD was less than 3%. Before and after honey Sunburn, the peak areas of chemical components changed, but the trends were not the same, and the changes of scopoletin showed a decreasing trend. Conclusion This article lays the foundation for further research on the changes of chemical composition before and after Sunburn.