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目的:观察YC-1对食管癌Eca109和胃癌SGC-7901细胞中Survivin表达的影响,探讨YC-1对食管癌和胃癌细胞生长的抑制作用。方法:Eca109和SGC-7901细胞分为对照组和YC-1实验组,细胞免疫组化和蛋白质印迹法检测YC-1对Survivin表达的影响,流式细胞仪检测YC-1对细胞凋亡和细胞周期的影响,MTT法检测YC-1对细胞增殖的影响。结果:YC-1抑制Eca109和SGC-7901细胞Sur-vivin的表达。5、10、20、30和40μmol/LYC-1对Eca109细胞48h增殖抑制率分别为(21.95±2.05)%、(30.69±2.59)%、(50.19±2.28)%、(61.17±2.09)%和(67.50±1.99)%;对SGC-7901细胞的抑制率分别为(9.32±0.61)%、(17.86±1.33)%、(36.95±2.01)%、(47.55±1.68)%和(56.01±1.12)%。10、20和30μmol/LYC-1作用于Eca109细胞,凋亡率分别为(14.1±0.81)%、(22.1±0.70)%和(24.6±1.01)%;作用于SGC-7901细胞,凋亡率为(15.9±0.75)%、(19.2±0.68)%、(25.4±0.95)%,P<0.05。0、20μmol/LYC-1作用于Eca109和SGC-7901细胞,S期比例为(21.45±0.91)%,(52.19±3.53)%;(24.41±2.12)%和(54.61±4.12)%,P<0.05。结论:YC-1下调Survivin的表达,诱导食管癌Eca109和胃癌SGC-7901细胞凋亡的发生并抑制细胞增殖,有可能对食管癌和胃癌具有潜在的治疗价值。
OBJECTIVE: To investigate the effect of YC-1 on the expression of Survivin in esophageal carcinoma Eca109 and gastric cancer SGC-7901 cells and to investigate the inhibitory effect of YC-1 on the growth of esophageal and gastric cancer cells. Methods: Eca109 and SGC-7901 cells were divided into control group and YC-1 experimental group. The effect of YC-1 on Survivin expression was detected by immunohistochemistry and Western blot. Flow cytometry was used to detect the apoptosis and Cell cycle, MTT assay YC-1 on cell proliferation. Results: YC-1 inhibited Survivin expression in Eca109 and SGC-7901 cells. The inhibitory rates of 5,10,20,30 and 40μmol / L YC-1 on proliferation of Eca109 cells at 48h were (21.95 ± 2.05)%, (30.69 ± 2.59)%, (50.19 ± 2.28)%, (61.17 ± 2.09)% and (67.50 ± 1.99)%, respectively. The inhibitory rates of SGC-7901 cells were (9.32 ± 0.61)%, (17.86 ± 1.33)%, (36.95 ± 2.01)%, (47.55 ± 1.68)% and (56.01 ± 1.12) %. The apoptotic rates of Eca109 cells treated with 10, 20 and 30 μmol / L YC-1 were (14.1 ± 0.81)%, (22.1 ± 0.70)% and (24.6 ± 1.01)%, respectively. The percentage of S phase was (21.45 ± 0.91)% in the S phase (15.9 ± 0.75)%, (19.2 ± 0.68)%, (25.4 ± 0.95)%, P <0.05.0 and 20μmol / LYC- ), (52.19 ± 3.53)%, (24.41 ± 2.12)% and (54.61 ± 4.12)% respectively, P <0.05. Conclusion: YC-1 down-regulates the expression of Survivin, induces the apoptosis of esophageal cancer cell line Eca109 and gastric cancer cell line SGC-7901, and inhibits cell proliferation. It may have potential therapeutic value for esophageal cancer and gastric cancer.