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在中国科学院植物研究所和区玉米研究所、区农学院的指导和帮助下,我们于1976年底对于玉米单倍体育种开始进行试验研究。1977年早造玉米接种15个材料,接种3,109管,出愈伤组织3块,其中“荣劳”2块,“靖西3号”1块(靖西3号长了根并出现了绿芽点,但因长期长不出苗而死亡)。晚造玉米接种3,430管,得愈伤组织494块,其中分化出绿芽(未成苗)148个,绿芽分化频率为38.4％。分化成绿苗192株,绿苗,绿芽总分化频率为68.3％。绿苗移植至土壤
With the guidance and help of the Institute of Botany, Chinese Academy of Sciences and the Regional Corn Research Institute and District Agricultural College, we started the pilot study on maize haploid breeding at the end of 1976. In early 1977, 15 maize inoculation materials were inoculated, 3,109 tubes were inoculated and 3 callus tissues were obtained, of which 2 were “Rong-lang”, 1 was “Jingxi 3” (Jingxi 3 was rooted and green shoots appeared Point, but died due to long-term emergence). 3,430 tubes of late-made maize were inoculated and 494 callus tissues were obtained, in which 148 green shoots (not mature) were differentiated and the frequency of green shoots differentiation was 38.4%. Divided into 192 green seedlings, green seedlings, green shoots the total frequency of differentiation was 68.3%. Green seedlings transplanted to the soil