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应用共生菌沃尔巴克氏体Wolbachia的wsp、ftsZ和16SrDNA基因特异引物,通过PCR扩增法对采自江西省南昌、九江和赣州等3地桔园内的东方钝绥螨、江原钝绥螨、尼氏真绥螨、巴氏新小绥螨、桔全爪螨和酢浆草如叶螨等6种常见螨类进行了检测。结果表明,在采自南昌桔园内的酢浆草如叶螨体内检测到Wolbachia感染,其wsp、ftsZ和16SrDNA基因长度为593、758和896bp,Genbank登录号依次为GQ162482、GQ162483和GQ162484,而其他5种螨体内均未检测出Wolbachia的感染;同时通过对酢浆草如叶螨体内感染的Wolbachia的wsp基因序列进行系统发育分析,得出其与B大组的Epo组的Wolbachia株十分相近,可能是相近或相同的株。
The Wsp, ftsZ and 16S rDNA gene-specific primers of Wolbachia were used to amplify the Amblyseius acuminatum and Atriplex mongolicus collected from the orange orchards in 3 sites of Nanchang, Jiujiang and Ganzhou in Jiangxi Province by PCR amplification. , Nigella spp., Pasteurella sylvestris, Citrus holothurica and Sorrel as spider mite were tested. The results showed that the wsp, ftsZ and 16S rDNA genes were 593, 758 and 896 bp in length, respectively. The GenBank accession numbers were GQ162482, GQ162483 and GQ162484, while the Wolbachia infection was detected in oxalis sp. The Wolbachia infection was not detected in the other five species of mites. The phylogenetic analysis of the Wsp gene sequence in Wolbachia infected with Oxaliphillum sp, such as spider mite, was very similar to that of the Wolbachia strain in the Epo group, , May be similar or identical strains.