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目的通过对静脉和球后2种途径接种白血病细胞建立模型的方法进行比较,探讨建立急性粒单核细胞性(粒单)白血病动物模型的有效方法。方法BALB/c小鼠分别经尾静脉和球后接种1×106WEHI-3细胞,观测其生存天数,光镜监测BALB/c小鼠外周血白细胞计数、未成熟粒单核细胞百分率及骨髓原始粒单核细胞百分率,电镜观测其骨髓白血病细胞形态。结果由静脉途径接种的小鼠生存时间要比由球后途径接种的小鼠明显延长,达到38.40±2.21d;其外周血白细胞计数和未成熟粒单核细胞百分率在第14天明显升高,分别为(19.24±1.73)×109/L和(6.24±0.85)%,并有上升的趋势;其骨髓原始粒单核细胞百分率亦升高明显,达到(41.3±4.90)%.由球后途径接种的小鼠其生存时间为16±2d,外周血白细胞计数、未成熟粒单核细胞百分率及骨髓原始粒单核细胞百分率一直处于正常范围内。结论WEHI-3细胞经静脉途径植入BALB/c小鼠体内,可形成典型的白血病模型,经球后途径接种则不能形成该模型。
OBJECTIVE: To establish an effective method for establishing animal model of acute myelomonocytic leukemia by comparing the methods of establishing leukemia cells by intravenous and post-ballooning leukemia cells. Methods BALB / c mice were inoculated with 1 × 106WEHI-3 cells via the caudal vein and the ball respectively to observe their survival days. The peripheral blood leukocyte count, Mononuclear cell percentage, electron microscope observation of myeloid leukemia cell morphology. Results The survival time of mice inoculated by intravenous route was significantly longer than that in mice vaccinated with the ball route (38.40 ± 2.21 days). The peripheral blood leukocyte count and the percentage of immature monocytes were significantly increased on the 14th day, (19.24 ± 1.73) × 109 / L and (6.24 ± 0.85)% respectively, and there was a trend of increase.The percentage of primary myelomonocytic mononuclear cells also increased significantly (41.3 ± 4.90)% Inoculated mice had a survival time of 16 ± 2 days, and peripheral blood leukocyte count, immature monocyte percentage and bone marrow mononuclear cell percentage were always in the normal range. Conclusion WEHI-3 cells can be injected into BALB / c mice intravenously to form typical leukemia model.