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目的比较两种巢氏PCR方法在检测二期梅毒患者血液标本中的价值。方法针对tpp47和pol A,分别建立两个巢氏PCR扩增全血中的梅毒螺旋体DNA。结果在353例梅毒阳性全血中,n PCR-tpp47和n PCR-pol A分别检出142例(40.2%)和132例(37.4%),结果差异有统计学意义(χ2=5.79,P<0.05);两种巢氏PCR在血清学试验高滴度(RPR≥1:8或TPPA≥1:2 560)组的检出率明显高于低滴度组(RPR<1:8或TPPA<1:2 560)。结论在检测二期梅毒全血标本Tp DNA时,n PCR-tpp47优于n PCR-pol A;巢氏PCR方法可作为梅毒血清学试验的补充试验。
Objective To compare the value of two nested PCR in detecting blood samples of patients with secondary syphilis. Methods Tpl47 and pol A were used to amplify the DNA of Treponema pallidum in whole blood by two nested PCR. Results In 353 cases of syphilis positive whole blood, 142 (40.2%) and 132 (37.4%) were detected by n PCR-tpp47 and n PCR-pol A respectively. The difference was statistically significant (χ2 = 0.05). The detection rates of two kinds of nested PCR in serological test with high titer (RPR≥1: 8 or TPPA≥1: 2 560) were significantly higher than those in low titer group (RPR <1: 8 or TPPA < 1: 2 560). Conclusion n PCR-tpp47 is better than n PCR-pol A in detection of Tp DNA in whole blood of second-stage syphilis. Nestin PCR can be used as a supplementary test for syphilis serological test.