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为了探讨水稻白叶枯病成株抗性是否与防卫基因相关 ,通过RT PCR研究了几种防卫基因PAL、LOX、PBZ1、Cht 1和PR1a的表达 ,同时也分析了PAL和LOX的酶活性变化 .结果表明 ,苗期PAL受病原菌诱导上调表达 ,而成株期病原菌和伤害均可诱导PAL上调表达 ,且成株期诱导表达强于苗期 .LOX在苗期和成株期都可受病原菌诱导表达 ,但成株期表达强于苗期且更持久 .PBZ1在苗期和成株期均受病原菌和伤害诱导表达 ,但成株期表达较苗期早且更强 .PR1a和Cht1没检测到期望的扩增片段 .PAL和LOX的酶活性分析结果与基因表达分析结果一致 .因此 ,PAL、LOX和PBZ1在苗期和成株期的表达差异可能与水稻白叶枯病成株抗性相关 .
In order to investigate whether the adult plant resistance to rice bacterial leaf blight is related to defense genes, the expression of several defense genes PAL, LOX, PBZ1, Cht 1 and PR1a were studied by RT-PCR. The changes of enzyme activities of PAL and LOX The results showed that PAL was up-regulated by pathogenic bacteria at seedling stage, while up-regulated expression of PAL was induced by pathogenic bacteria and injury at adult stage, and its expression at adult stage was stronger than that at seedling stage.LOX could be affected by pathogen at seedling and adult stage But the expression in the adult stage was stronger than that in the seedling stage.PBZ1 was induced by pathogen and injury at seedling stage and adult stage but was earlier and stronger at adult stage than that at seedling stage.PR1a and Cht1 were not detected To the desired amplified fragment.PAL and LOX enzyme activity analysis results and gene expression analysis of the results.Therefore, PAL, LOX and PBZ1 at seedling and adult stage of the difference may be resistant to rice bacterial blight Related.