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白色念球菌在机体内形成厚膜孢子的例子尚未见文献报导,笔者曾从一个病人的新鲜痰液中发现有白色念珠菌的典型厚膜孢子,并经培养检查证实。病者的痰呈黏液性混有粥渣样的白色粒团,涂片检查用萃兰氏法染色,又以棉兰液及10%氢氧化钾液分别做成湿片标本,从菌丝与孢子结构,立即可以诊断是白色念珠菌,这些大而圆的厚膜孢子(直径6～8微米)有双重壁和包涵粒体的存在。另外尚有很多芽生孢子和假菌丝,玻片标本的制作是用接种环将带白色小片的痰液轻铺于玻片上;不加反复涂抹以避免捣乱微生物的形态结构。从许多革兰氏染色涂片标本以及棉兰湿片标本所得的印象:菌体结构在排列上与在玉蜀黍培基上的不同,在玉蜀黍琼脂培基上菌丝较短,且不会纠缠成团,其芽生孢子每呈球形集簇环绕菌丝生长,但是在痰液标本中,多数菌丝(假菌丝)很长,亦有互相纠缠成团的。芽生孢子不呈球形集簇,不环
Candida albicans in the body to form thick film spore examples have not been reported in the literature, the author had a patient’s fresh sputum found in the typical thick conidia of Candida albicans, and confirmed by the culture examination. The patient’s sputum mucus was mixed with porridge-like white particles, smear examination with Portland’s staining, but also to cotton and 10% solution of potassium hydroxide were made wet specimens, from mycelium and Spore structure, immediately diagnosed as Candida albicans, these large, round, thick-film spores (6-8 μm in diameter) have the presence of double walls and inclusion bodies. In addition, there are a lot of spores and pseudohyphae, slide specimens were made by inoculating the ring with a white piece of sputum on the slides on the glass; without repeated smear to avoid disrupting the morphological structure of microorganisms. The impression obtained from many Gram-stained smears and samples of Medaka: The mycelium is arranged in a different arrangement on Zea mays cultivars with shorter hyphae on Zea mays agar and will not entangle However, in the sputum samples, most hyphae (pseudohyphae) are very long and entangled with each other. Bacillus spores do not spherical clusters, not ring