目的研究托瑞米芬(TOR)联合吉西他滨(GEM)对人肺癌株A549的影响,为肺癌的临床治疗提供新的方向。方法用MTT比色法检测各组药物对细胞生长的抑制作用,用HE染色观察用药后细胞形态,用细胞凋亡试剂盒检测细胞凋亡率。结果托瑞米芬能直接抑制肺腺癌细胞A549的生长,低剂量托瑞米芬(5、10μmol/L)与吉西他滨联用后的抑制率较单用吉西他滨明显提高,并且随药物浓度增高而增高。联合用药组较单用化疗药物组凋亡率提高,而单用托瑞米芬对细胞的凋亡率与对照组比无明显变化。结论托瑞米芬可抑制肺腺癌细胞A549的生长,但不能直接促进细胞凋亡;托瑞米芬与吉西他滨有明显的协同作用。 Objective To study the effect of TOR combined with gemcitabine (GEM) on human lung cancer A549 cells and to provide a new direction for the clinical treatment of lung cancer. Methods MTT assay was used to detect the inhibitory effect of each drug on cell growth. The cell morphology was observed by HE staining and the apoptosis rate was detected by using the apoptosis kit. Results Toremifene can directly inhibit the growth of lung adenocarcinoma A549 cells. Compared with gemcitabine alone, the inhibitory rate of toremifene combined with gemcitabine (5,10 μmol / L) was significantly increased, and with the increase of drug concentration Increase. Compared with the chemotherapy alone group, the apoptosis rate of combination group increased, while the apoptosis rate of toremifene alone group had no significant change compared with the control group. Conclusion Toremifene can inhibit the growth of lung adenocarcinoma A549 cells, but not directly promote the apoptosis of cells. Toremifene has a synergistic effect with gemcitabine.