为进一步确定ras基因突变与肿瘤恶性表型的关系,我们构建了一系列的v-ras突变体并将其导入NIH 3T3细胞,观察这些细胞的恶性表型,如致瘤性,转移能力及细胞膜组织相容性抗原(MHC-I)的表达水平。实验证明不是全部具有形态转化的细胞都能在正常的Balb/c小鼠致瘤和形成远端转移。ras基因不仅能影响细胞的形态、致瘤性、转移能力,而且还通过调节MHC-I的表达水平来影响ras转化细胞的恶性表型。 To further determine the relationship between ras gene mutations and the malignant phenotype of tumors, we constructed a series of v-ras mutants and introduced them into NIH 3T3 cells to observe the malignant phenotypes of these cells, such as tumorigenicity, metastatic ability, and cell membranes. The expression level of histocompatibility antigen (MHC-I). Experiments have shown that not all cells with morphological transformation can be tumorigenic and form distant metastases in normal Balb/c mice. The ras gene not only affects the cell morphology, tumorigenicity, metastasis capacity, but also affects the malignant phenotype of ras transformed cells by regulating the expression level of MHC-I.