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目的研究感染HCV的无症状献血者基因型和1b基因型的核心区氨基酸置换情况。方法用巢式PCR方法扩增91个HCV RNA阳性献血者标本,根据直接测序得到的序列用Mega6.0软件进行序列比对和进化树构建,分析基因型和氨基酸置换情况。结果在78个测序结果清晰的样品中,66个为基因型1b(84.6%),其他基因型为1a(1,1.3%)、2a(7,8.9%)、3b(2,2.6%)和6a(2,2.6%)。62个1b样品的核心区蛋白(aa68-120)发生17处氨基酸置换。第70位R70Q、R70P和R70H各1例;第91位L91M置换非常普遍,为87.9%。结论本研究中献血者感染的HCV基因型主要为1型,尤其是1b为优势基因型。首次发现中国献血者感染的HCV核心区蛋白的氨基酸置换具有独特性。
Objective To study the core amino acid substitutions of genotypes 1b and 1b in asymptomatic blood donors infected with HCV. Methods A total of 91 HCV RNA positive blood donors were amplified by nested PCR. According to the sequences obtained by direct sequencing, the sequences were aligned and phylogenetic tree was constructed by Mega6.0 software to analyze the genotypes and amino acid substitutions. Results Among the 78 samples with clear sequencing results, 66 were genotype 1b (84.6%) and other genotypes were 1a (1, 1.3%), 2a (7,8.9%), 3b 6a (2, 2.6%). Seventeen amino acid substitutions occurred in the core region of 62 1b samples (aa68-120). The 70th R70Q, R70P and R70H each had 1 case; the 91st L91M replacement was very common, 87.9%. Conclusion The HCV genotypes of blood donors in this study were predominantly type 1, especially 1b being the predominant genotype. For the first time, it was found that the amino acid substitutions of HCV core protein in Chinese blood donors are unique.