大豆根瘤菌的田间侵染过程及其影响因素的分析,对提高根瘤菌接种效果有密切关系。本试验对我省几个大豆品种不同生育期的根瘤进行了测试,并选用对我省大豆品种感染性较强的菌系113—2菌株进行了接种效果比较试验和较大面积的示范应用,获得增产17～38％的增产效果,每亩增产大豆20斤以上。方法与结果田间大豆根瘤菌侵染动态分析,用凝集反应测定。根瘤样品用50％甘油盐水保藏。抗血清滴度用其效价的上一个滴度,抗原用0.85％NaCl溶液在研钵中研磨。抗原一抗体加好后置37℃温箱或水浴中过夜,第二天 Rhizobium soybean field infection process and its influencing factors analysis, to improve the effect of Rhizobia inoculation are closely related. This experiment tested several nodules at different growing stages in our province and compared the inoculation effect of 113-2 strain which is highly infecting soybean in our province with a large area for demonstration application. Gain 17 ~ 38% yield increase effect, yield more than 20 pounds of soybean per acre. Methods and Results Dynamic analysis of soybean rhizobia infestation in field and determination by agglutination. Nodule samples were stored in 50% glycerol saline. Antisera were titrated with the titer of their titer and the antigen was ground in a mortar with 0.85% NaCl solution. An anti-antibody plus post 37 ℃ incubator or water bath overnight, the next day