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目的探讨妊娠期高血压疾病(HDCP)患者氧自由基对红细胞膜补体受体1型分子(CR1)数量表达水平变化(即活性变化)的影响。方法采用酶联免疫(ELISA)法测定红细胞CR1分子的数量;同时采用化学比色法检测血浆丙二醛(MDA)、超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GSH-PX),观察其水平的变化,并进行相关性分析。结果①患者红细胞CR1分子表达水平显著低于对照组(P<0.01);②患者SOD和GSH-PX的水平显著下降,MDA的水平显著上升;③红细胞CR1分子表达水平与SOD和GSH-PX的水平呈显著的正相关(r=0·804、P<0·01;r=0·838、P<0·01);与MDA的水平呈显著的负相关(r=-0·760、P<0·01)。结论红细胞膜极易产生脂质过氧化作用,妊娠期高血压疾病患者自由基损伤的程度可直接影响红细胞膜的功能,特别是红细胞膜CR1分子表达降低,至患者红细胞免疫粘附(RCIA)功能降低,提示减少机体氧自由基的产生,提高患者RCIA功能是十分必要的。
Objective To investigate the effects of oxygen free radicals on the expression of erythrocyte membrane complement receptor type 1 (CR1) molecules in patients with gestational hypertension (HDCP). Methods The number of erythrocyte CR1 was determined by enzyme-linked immunosorbent assay (ELISA). At the same time, the contents of malondialdehyde (MDA), superoxide dismutase (SOD) and glutathione peroxidase (GSH) -PX), observed the level of changes, and correlation analysis. Results The level of CR1 in erythrocytes was significantly lower than that in the control group (P <0.01). The level of SOD and GSH-PX was significantly decreased and the level of MDA was significantly increased. The expression of CR1 in erythrocytes was correlated with the level of SOD and GSH-PX (R = 0.804, P <0.01; r = 0.3838, P <0.01), and had a significant negative correlation with the level of MDA (r = -0.760, P <0 · 01). Conclusion Erythrocyte membrane is prone to produce lipid peroxidation. The degree of free radical damage in patients with hypertensive disorders during pregnancy may directly affect the function of erythrocyte membrane, especially the decrease of erythrocyte membrane CR1 molecule expression, to the patients with erythrocyte immune adhesion (RCIA) function Reduce, suggesting that reduce the body’s production of oxygen free radicals, improve patient RCIA function is very necessary.