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①目的观察五子降糖方对骨骼肌细胞胰岛素抵抗的影响,探讨其改善胰岛素抵抗作用的可能机制。②方法对骨骼肌细胞应用5×10-7 mol/L胰岛素干预12小时建立胰岛素抵抗模型,应用低、中、高剂量(25μg/mL,100μg/mL,400μg/mL)五子降糖方对造模成功的骨骼肌细胞进行干预,同时设正常组、模型组,药物干预24小时后用葡萄糖氧化酶法测定骨骼肌细胞葡萄糖消耗量;③结果五子降糖方各组与模型组比较葡萄糖消耗量高于模型组(P<0.01),高剂量组与正常组比较,葡萄糖消耗量无统计学差异(P>0.05),中剂量组与正常组比较,葡萄糖消耗量低于正常组(P<0.05),低剂量组葡萄糖消耗量显著低于正常组(P<0.01);④结论五子降糖方可以改善骨骼肌细胞胰岛素抵抗模型的葡萄糖代谢。
①Objective To observe the effect of Wuzi Jiangtang Recipe on insulin resistance of skeletal muscle cells and to explore its possible mechanism of improving insulin resistance. Methods Insulin resistance was induced in skeletal muscle cells by 5 × 10-7 mol / L insulin for 12 hours. Low, medium and high doses (25μg / mL, 100μg / mL, 400μg / mL) Model of skeletal muscle cells intervention, at the same time set the normal group, model group, 24 hours after drug intervention, glucose oxidase method was used to determine the glucose consumption of skeletal muscle cells; ③ Results Wuzi Hypoglycemic group compared with the model group, glucose consumption (P <0.01). There was no significant difference in glucose consumption between the high-dose group and the normal group (P> 0.05). Compared with the normal group, the medium-dose glucose consumption was lower than that of the normal group (P <0.05) ), And the glucose consumption in the low-dose group was significantly lower than that in the normal group (P <0.01); ④Conclusion Wubi Hypoglycemic prescription can improve the glucose metabolism of skeletal muscle insulin resistance model.