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【目的】探讨蒜素(Allicin)对神经样 PC12细胞 Rho 亚家族蛋白 A(RhoA)活性和轴突生长的影响。【方法】体外培养神经样PC12细胞,RhoA激动剂溶血磷脂酸(1 ysophosphatidic acid,LPA)诱导 RhoA活化,并使用 Allicin干预,Western blot检测 RhoA、相关卷曲螺旋形成蛋白激酶1(R0ck1)、相关卷曲螺旋形成蛋白激酶2(Rock2)、轴突生长蛋白神经微丝(NF)、突触蛋白(synapsin)、突触素(synaptophysin)表达并比较。【结果】LPA作用诱导 RhoA活化,RhoA、ROCK1、ROCK2过表达,NF、synapsin、synaptophysin 表达显著降低。Allicin干预处理后,RhoA 活化被抑制,RhoA、ROCK1、ROCK2表达下调；恢复 NF、synapsin、synapto-physin表达。【结论】Allicin干预能有效缓解 LPA 处理 PC12细胞诱导的 RhoA 活化和对轴突生长的抑制；提示 Allicin可通过抑制 RhoA的活化促进轴突生长。“,”Obj ective]To observe the effects of allicin on RhoA activity and axonal growth in neuron-like PC1 2 cells and to explore the relationship between allicin-promoting PC1 2 cells axon growth and RhoA/ROCK signaling activity.[Methods]The effects of Allicin on the growth of PC1 2 cells were detected by MTT assay. The low,medium,and high concentrations of Allicin,which had no effect on the growth of PC1 2 cells,were selected.PC1 2 cells were cultured in medium with LPA to induce the activation of RhoA/ROCK signaling pathway and intervened with low,medium,and high concentrations of Allicin.Western blotting was used to detect the expression of ROCK1,ROCK2,RhoA,NF,synapsin and synaptophysin.[Results]The three Alli-cin concentrations (10 ng/ml,100 ng/ml and 1μg/ml)had no effect on the growth of PC12 cells.The expres-sion of RhoA,ROCK1 and ROCK2 were significantly increased and the expression of NF,synapsin and syn-aptophysin were dramatically reduced in PC1 2 cells treatment with LPA.Allicin could effectively reduced RhoA,ROCK1 and ROCK2 expression and recover the expression of NF,synapsin and synaptophysin.[Con-clusion]Allicin intervention can effectively alleviate RhoA activation and inhibitory effects for axon growth in-duced by LPA.Allicin can promote the growth of axons through inhibiting the activation of RhoA.