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目的 :研究纤维粘连蛋白在牙髓修复中的免疫定位和分布特征。方法 :在大鼠第一磨牙制备单面洞 ,分别观察 3d、15d和 30d后修复性牙本质形成情况。免疫组织化学技术检测纤维粘连蛋白的免疫反应。结果 :术后 3d ,修复性牙本质尚未形成。牙髓内成牙本质细胞呈弱阳性染色 ,前期牙本质也为弱阳性。术后 15d ,可见修复性牙本质形成 ,成牙本质细胞样细胞和牙髓成纤维细胞免疫染色呈阳性。术后 30d ,已形成的修复性牙本质内纤维粘连蛋白染色为强阳性 ,特别集中于修复性牙本质与牙髓的交界面 ,成牙本质细胞样细胞染色呈强阳性。结论 :纤维粘连蛋白可能在修复性牙本质形成中起重要调节作用。
Objective: To study the immunolocalization and distribution of fibronectin in pulp restoration. Methods: One-sided holes were prepared in the first molar of rats, and the formation of reparative dentin was observed after 3d, 15d and 30d respectively. Immunohistochemical detection of fibronectin immune response. Results: Repair dentin has not formed after 3d. Odontoblasts within the pulp cells were weakly positive staining, early dentin is also weakly positive. Fifteen days after operation, reparative dentin formation, odontoblast-like cells and pulp fibroblast immunostaining were positive. At 30 days after operation, the reparative dentin fibronectin staining was strongly positive, especially in the interface between reparative dentin and dental pulp. The staining of odontoblast-like cells was strongly positive. Conclusion: Fibronectin may play an important role in the formation of reparative dentin.